Assignment of the human neuropeptide Y gene to chromosome 7p15.1 by nonisotopic in situ hybridization
- Women`s and Children`s Hospital, Adelaide (Australia)
- Garvan Inst. of Medical Research, Sydney (Australia); and others
A 15-kb genomic fragment containing the whole human NPY gene in the vector {lambda}GEM-11 was nick-translated with biotin-14-dATP and hybridized in situ at a final concentration of 5 ng/{mu}l to metaphases from two males. The fluorescence in situ hybridization method was modified from that previously described in that chromosomes were stained before analysis with both propidium iodide (as counterstain) and DAPI (for chromosome identification). Images of metaphase preparations were captured by a CCD camera and computer enhanced.
- OSTI ID:
- 249978
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 26; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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