Assignment of the tyrosinase-related protein-2 gene (TYRP2) to human chromosome 13q31-q32 by fluorescence in situ hybridization: Extended synteny with mouse chromosome 14

Journal Article · Sun May 01 04:00:00 EDT 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1266· OSTI ID:7029692

Sturm, R A ^[1]; Baker, E; Sutherland, G R ^[2]

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1. Univ. of Queensland, Brisbane (Australia)
2. Centre for Medical Genetics, North Adelaide (Australia)

A recombinant human genomic liver DNA [lambda]-phage library was screened with the insert of the pHuTRP-2 cDNA clone to isolate a series of bacteriophage with inserts spanning the human TYRP2 gene. One of the [lambda]-phage clones ([lambda]HuT-YRP2-7) containing a 2-kb HindIII fragment with the 5[prime] exon sequence of the cDNA as determined by sequence analysis was used for the gene localization study. DNA prepared from the phage by Qiagen chromatography was nick-translated with biotin-14-dATP and hybridized in situ at a final concentration of 5 ng/[mu]l to metaphases from two normal males. The fluorescence in situ hybridization method was modified from that previously described in that chromosomes were stained before analysis with both propidium iodide as counterstain and DAPI for chromosome identification. Twenty metaphases from the first normal male were examined for fluorescent signal. All of these metaphases showed signal on one or both chromatids of chromosome 13 in the region 13q31-q33; 88% of this signal was at the interface of bands 13q31-q32. There was a total of four nonspecific background dots observed in these 20 metaphases. A similar result was obtained from hybridization of the probe to 20 metaphases from the second normal male (data not shown). This region has also been shown to contain the propionyl coenzyme A carboxylase [alpha]-chain gene by in situ hybridization. The localization of the TYRP2 locus to human chromosome 13q31-q32 extends the syntenic region of chromosome 13 with mouse chromosome 14. 15 refs., 1 fig.

• OSTI ID: 7029692
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 21:1; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
BIOTECHNOLOGY
CHROMOSOMES
DNA HYBRIDIZATION
GENETIC ENGINEERING
GENETIC MAPPING
HUMAN CHROMOSOME 13
HUMAN CHROMOSOMES
HYBRIDIZATION
HYDROXY COMPOUNDS
MAPPING
MELANIN
NUCLEIC ACID HYBRIDIZATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
SYNTHESIS