Confocal Raman Microscopy for Investigating Synthesis and Characterization of Individual Optically Trapped Vinyl-Polymerized Surfactant Particles

Schaefer, Jonathan J.; Crawford, Alexis C.; Porter, Marc D.; Harris, Joel M.

doi:10.1366/13-07337

Confocal Raman Microscopy for Investigating Synthesis and Characterization of Individual Optically Trapped Vinyl-Polymerized Surfactant Particles

Journal Article · Sun Jun 01 00:00:00 EDT 2014 · Applied Spectroscopy

DOI:https://doi.org/10.1366/13-07337· OSTI ID:2480565

Schaefer, Jonathan J. ^[1]; Crawford, Alexis C. ^[2]; Porter, Marc D. ^[2]; Harris, Joel M. ^[2]

University of Utah, Salt Lake City, UT (United States); University of Utah
University of Utah, Salt Lake City, UT (United States)

Small polymeric particles are increasingly employed as adsorbent materials, as molecular carriers, as delivery vehicles, and in preconcentration applications. The rational development of these materials requires in situ methods of analysis to characterize their synthesis, structure, and applications. Optical-trapping confocal Raman microscopy is a spectroscopic method capable of acquiring information at several stages of the development of such dispersed particulate materials. In the present study, an example material is developed and tested using confocal Raman microscopy for characterization at each stage of the process. Specifically, the method is used to investigate the synthesis, structure, and applications of individual polymeric surfactant particles produced by the vinyl polymerization of sodium 11-acrylamidoundecanoate (SAAU). The kinetics of polymerization can be monitored over time by measuring the loss of the acrylamide C=C functional groups using confocal Raman microscopy of particles optically trapped by the excitation laser, where, within the limits of detecting the vinyl functional group, the complete polymerization of the SAAU monomer was achieved. The polymerized SAAU particles are spherical, and they exhibit uniform access to water throughout their structure, as tested by the penetration of heavy water (D2O) and collection of spatially resolved Raman spectra from the interior of the particle. These porous particles contain hydrophobic domains that can be used to accumulate molecules for adsorption or carrier applications. This property was tested by using confocal Raman microscopy to measure the accumulation equilibria and kinetics of a model compound, dioxybenzone. The partitioning of this compound into the polymer surfactant could be determined on a quantitative basis using relative scattering cross sections of the SAAU monomer and the adsorbate. Furthermore, the study points out the utility of optical-trapping confocal Raman microscopy for investigating the synthesis, structure, and potential carrier applications of polymeric particle materials.

View Accepted Manuscript (DOE)

Research Organization:: University of Utah, Salt Lake City, UT (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); National Science Foundation MRSEC program

Grant/Contract Number:: FG03-93ER14333

OSTI ID:: 2480565

Journal Information:: Applied Spectroscopy, Journal Name: Applied Spectroscopy Journal Issue: 6 Vol. 68; ISSN 0003-7028

Publisher:: Society for Applied SpectroscopyCopyright Statement

Country of Publication:: United States

Language:: English

References (33)

Deuterium substitution and fluorescence studies on polymer hydrogels and complexes Shirota, Hideaki; Horie, Kazuyuki Macromolecular Symposia, Vol. 207, Issue 1 https://doi.org/10.1002/masy.200450308	journal	February 2004
Pressure induced changes in the Raman spectra of liquidn-alkanes and perfluoro-n-alkanes Schwickert, H.; Strobl, G. R.; Eckel, R. Colloid and Polymer Science, Vol. 260, Issue 6 https://doi.org/10.1007/BF01422590	journal	June 1982
Applications of the optical trapping technique to analyze chemical reactions in single emulsion particles Urlaub, E.; Lankers, M.; Hartmann, I. Analytical and Bioanalytical Chemistry, Vol. 355, Issue 3-4 https://doi.org/10.1007/s0021663550329	journal	June 1996
Characterization of polymeric vesicles of poly(sodium 11-acrylamidoundecanoate) in water Nayak, Rati Ranjan; Roy, Sumita; Dey, Joykrishna Colloid and Polymer Science, Vol. 285, Issue 2 https://doi.org/10.1007/s00396-006-1554-z	journal	September 2006
Raman investigation of styrene polymerization in single optically trapped emulsion particles Urlaub, E.; Lankers, M.; Hartmann, I. Chemical Physics Letters, Vol. 231, Issue 4-6 https://doi.org/10.1016/0009-2614(94)01297-0	journal	December 1994
Raman spectroscopy of octadecylsilane stationary phase conformational order Pemberton, Jeanne E.; Ho, Mankit; Orendorff, Christopher J. Journal of Chromatography A, Vol. 913, Issue 1-2 https://doi.org/10.1016/S0021-9673(01)00569-6	journal	April 2001
Organosilicate-polymer drug delivery systems: controlled release and enhanced mechanical properties Cypes, Stephen H.; Saltzman, W. Mark; Giannelis, Emmanuel P. Journal of Controlled Release, Vol. 90, Issue 2 https://doi.org/10.1016/S0168-3659(03)00133-0	journal	June 2003
Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles Pinto Reis, Catarina; Neufeld, Ronald J.; Ribeiro,, António J. Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 2, Issue 1 https://doi.org/10.1016/j.nano.2005.12.003	journal	March 2006
Confocal Raman Microscopy for Monitoring Chemical Reactions on Single Optically Trapped, Solid-Phase Support Particles Houlne, Michael P.; Sjostrom, Christopher M.; Uibel, Rory H. Analytical Chemistry, Vol. 74, Issue 17 https://doi.org/10.1021/ac020325t	journal	July 2002
Optical-Trapping Raman Microscopy Detection of Single Unilamellar Lipid Vesicles Cherney, Daniel P.; Conboy, John C.; Harris, Joel M. Analytical Chemistry, Vol. 75, Issue 23 https://doi.org/10.1021/ac034838r	journal	December 2003
Spatially Resolved Analysis of Small Particles by Confocal Raman Microscopy: Depth Profiling and Optical Trapping Bridges, Travis E.; Houlne, Michael P.; Harris, Joel M. Analytical Chemistry, Vol. 76, Issue 3 https://doi.org/10.1021/ac034969s	journal	February 2004
Optical Trapping of Unilamellar Phospholipid Vesicles: Investigation of the Effect of Optical Forces on the Lipid Membrane Shape by Confocal-Raman Microscopy Cherney, Daniel P.; Bridges, Travis E.; Harris, Joel M. Analytical Chemistry, Vol. 76, Issue 17 https://doi.org/10.1021/ac0492620	journal	September 2004
Detection of Drug−Membrane Interactions in Individual Phospholipid Vesicles by Confocal Raman Microscopy Fox, Christopher B.; Horton, Robert A.; Harris, Joel M. Analytical Chemistry, Vol. 78, Issue 14 https://doi.org/10.1021/ac0605290	journal	July 2006
Quantitative Confocal Raman Microscopy Study of Ion-Interaction Retention at Reversed-Phase Chromatographic Interfaces Gasser-Ramirez, Jennifer L.; Harris, Joel M. Analytical Chemistry, Vol. 82, Issue 13 https://doi.org/10.1021/ac100781p	journal	June 2010
Confocal Raman Microscopy of pH-Gradient-Based 10 000-Fold Preconcentration of Compounds within Individual, Optically Trapped Phospholipid Vesicles Myers, Grant A.; Harris, Joel M. Analytical Chemistry, Vol. 83, Issue 15 https://doi.org/10.1021/ac2012152	journal	August 2011
Trypsin Immobilization on Hairy Polymer Chains Hybrid Magnetic Nanoparticles for Ultra Fast, Highly Efficient Proteome Digestion, Facile ¹⁸O Labeling and Absolute Protein Quantification Qin, Weijie; Song, Zifeng; Fan, Chao Analytical Chemistry, Vol. 84, Issue 7 https://doi.org/10.1021/ac2029216	journal	March 2012
Confocal Raman Microscopy Probing of Temperature-Controlled Release from Individual, Optically-Trapped Phospholipid Vesicles Schaefer, Jonathan J.; Ma, Chaoxiong; Harris, Joel M. Analytical Chemistry, Vol. 84, Issue 21 https://doi.org/10.1021/ac302346n	journal	September 2012
Enhancing Cosmetics No authors listed Chemical & Engineering News Archive, Vol. 90, Issue 20 https://doi.org/10.1021/cen-09020-cover	journal	May 2012
Engineered Polymeric Nanoparticles for Soil Remediation Tungittiplakorn, Warapong; Lion, Leonard W.; Cohen, Claude Environmental Science & Technology, Vol. 38, Issue 5 https://doi.org/10.1021/es0348997	journal	January 2004
Viscosity and Density of H ₂ O + NaCl + CaCl ₂ and H ₂ O + KCl + CaCl ₂ at 298.15 K Zhang, Hai-Lang; Chen, Geng-Hua; Han, Shi-Jun Journal of Chemical & Engineering Data, Vol. 42, Issue 3 https://doi.org/10.1021/je9602733	journal	May 1997
Quantitative Correlation of Raman Spectral Indicators in Determining Conformational Order in Alkyl Chains Orendorff, Christopher J.; Ducey, Michael W.; Pemberton, Jeanne E. The Journal of Physical Chemistry A, Vol. 106, Issue 30 https://doi.org/10.1021/jp014311n	journal	July 2002
Detecting Phase Transitions in Phosphatidylcholine Vesicles by Raman Microscopy and Self-Modeling Curve Resolution Fox, Christopher B.; Uibel, Rory H.; Harris, Joel M. The Journal of Physical Chemistry B, Vol. 111, Issue 39 https://doi.org/10.1021/jp0735886	journal	October 2007
Self-organization and Microstructures of Sodium 11-Acrylamidoundecanoate in Water Roy, Sumita; Dey, Joykrishna Langmuir, Vol. 19, Issue 23 https://doi.org/10.1021/la0348113	journal	October 2003
Local Order in Liquid n -Alkanes: Evidence from Raman Spectroscopic Study Brambilla, Luigi; Zerbi, Giuseppe Macromolecules, Vol. 38, Issue 8 https://doi.org/10.1021/ma047390k	journal	April 2005
Recent advances in stimuli-responsive degradable block copolymer micelles: synthesis and controlled drug delivery applications Zhang, Qian; Re Ko, Na; Kwon Oh, Jung Chemical Communications, Vol. 48, Issue 61 https://doi.org/10.1039/c2cc32408c	journal	January 2012
Vibrational spectra and carbon–hydrogen stretching mode assignments for a series of n-alkyl carboxylic acids Hill, Ian R.; Levin, Ira W. The Journal of Chemical Physics, Vol. 70, Issue 2 https://doi.org/10.1063/1.437517	journal	January 1979
Raman band profiles and mobility of polymethylene chains Cho, Yonghae; Kobayashi, Masamichi; Tadokoro, Hiroyuki The Journal of Chemical Physics, Vol. 84, Issue 8 https://doi.org/10.1063/1.449989	journal	April 1986
Synthesis and Polymerization of Surface-Active Sodium Acrylamidoundecanoate Yeoh, K. W.; Chew, C. H.; Can, L. M. Journal of Macromolecular Science: Part A - Chemistry, Vol. 26, Issue 4 https://doi.org/10.1080/00222338908052001	journal	April 1989
Raman spectroscopy of optically trapped particles Petrov, D. V. Journal of Optics A: Pure and Applied Optics, Vol. 9, Issue 8 https://doi.org/10.1088/1464-4258/9/8/S06	journal	July 2007
Drug Delivery Systems: Entering the Mainstream Allen, T. M. Science, Vol. 303, Issue 5665 https://doi.org/10.1126/science.1095833	journal	March 2004
Confocal Raman Microscopy of Optical-Trapped Particles in Liquids Cherney, Daniel P.; Harris, Joel M. Annual Review of Analytical Chemistry, Vol. 3, Issue 1 https://doi.org/10.1146/annurev-anchem-070109-103404	journal	June 2010
Temperature-Controlled Confocal Raman Microscopy to Detect Phase Transitions in Phospholipid Vesicles Fox, Christopher B.; Myers, Grant A.; Harris, Joel M. Applied Spectroscopy, Vol. 61, Issue 5 https://doi.org/10.1366/000370207780807786	journal	May 2007
Confocal Raman Microspectroscopy Using a Stigmatic Spectrograph and CCD Detector Williams, K. P. J.; Pitt, G. D.; Batchelder, D. N. Applied Spectroscopy, Vol. 48, Issue 2 https://doi.org/10.1366/0003702944028407	journal	February 1994

Similar Records

Confocal Raman Microscopy of Optical-Trapped Particles in Liquids

Journal Article · Mon Mar 08 23:00:00 EST 2010 · Annual Review of Analytical Chemistry · OSTI ID:2480574

Spatial Filtering of a Diode Laser Beam for Confocal Raman Microscopy

Journal Article · Wed Apr 01 00:00:00 EDT 2015 · Applied Spectroscopy · OSTI ID:2480572

Confocal Raman Microscopy for in Situ Detection of Solid-Phase Extraction of Pyrene into Single C₁₈–Silica Particles

Journal Article · Mon Jan 06 23:00:00 EST 2014 · Analytical Chemistry · OSTI ID:2480571

Related Subjects

36 MATERIALS SCIENCE
Adsorption
Confocal Raman microscopy
Optical trapping
Polymer materials

Confocal Raman Microscopy for Investigating Synthesis and Characterization of Individual Optically Trapped Vinyl-Polymerized Surfactant Particles

Citation Formats

References (33)

Similar Records

Related Subjects