Spatial Filtering of a Diode Laser Beam for Confocal Raman Microscopy

Kitt, Jay P.; Bryce, David A.; Harris, Joel M.

doi:10.1366/14-07671

Spatial Filtering of a Diode Laser Beam for Confocal Raman Microscopy

Journal Article · Wed Apr 01 00:00:00 EDT 2015 · Applied Spectroscopy

DOI:https://doi.org/10.1366/14-07671· OSTI ID:2480572

Kitt, Jay P. ^[1]; Bryce, David A. ^[2]; Harris, Joel M. ^[2]

University of Utah, Salt Lake City, UT (United States); University of Utah
University of Utah, Salt Lake City, UT (United States)

With the development of single-longitudinal mode diode lasers, there has been an increase in using these sources for Raman spectroscopy. This is largely due to the cost-effectiveness of diode lasers, which offer savings not only in initial capital cost, but also electrical, cooling, and replacement costs over time, when compared with ion lasers. Here, the use of diode-lasers in confocal Raman microscopy has remained a challenge, however, due to poor transverse beam quality. In this work, we present the design and implementation of a simple spatial filter capable of adapting a single-mode diode laser source to confocal Raman microscopy, yielding comparable spatial resolution as a gas-ion laser beam for profiling and optical-trapping applications. For profiling applications, spatial filtering improved x,y resolution of the beam by a factor 10, which in turn increased optical-trapping forces by ~90 times and yielded sevenfold greater Raman scattering signal intensity from an optically trapped phospholipid vesicle.

View Accepted Manuscript (DOE)

Research Organization:: University of Utah, Salt Lake City, UT (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); NSF-IGERT Program

Grant/Contract Number:: FG03-93ER14333

OSTI ID:: 2480572

Journal Information:: Applied Spectroscopy, Journal Name: Applied Spectroscopy Journal Issue: 4 Vol. 69; ISSN 0003-7028

Publisher:: Society for Applied SpectroscopyCopyright Statement

Country of Publication:: United States

Language:: English

References (21)

Laser Fundamentals Silfvast, William T. https://doi.org/10.1017/CBO9780511616426	book	January 2004
Optical-Trapping Raman Microscopy Detection of Single Unilamellar Lipid Vesicles Cherney, Daniel P.; Conboy, John C.; Harris, Joel M. Analytical Chemistry, Vol. 75, Issue 23 https://doi.org/10.1021/ac034838r	journal	December 2003
Spatially Resolved Analysis of Small Particles by Confocal Raman Microscopy: Depth Profiling and Optical Trapping Bridges, Travis E.; Houlne, Michael P.; Harris, Joel M. Analytical Chemistry, Vol. 76, Issue 3 https://doi.org/10.1021/ac034969s	journal	February 2004
Optical Trapping of Unilamellar Phospholipid Vesicles: Investigation of the Effect of Optical Forces on the Lipid Membrane Shape by Confocal-Raman Microscopy Cherney, Daniel P.; Bridges, Travis E.; Harris, Joel M. Analytical Chemistry, Vol. 76, Issue 17 https://doi.org/10.1021/ac0492620	journal	September 2004
Detection of Glutamate in Optically Trapped Single Nerve Terminals by Raman Spectroscopy Ajito, Katsuhiro; Han, Chunxi; Torimitsu, Keiichi Analytical Chemistry, Vol. 76, Issue 9 https://doi.org/10.1021/ac049969m	journal	March 2004
Confocal Raman Microscopy of pH-Gradient-Based 10 000-Fold Preconcentration of Compounds within Individual, Optically Trapped Phospholipid Vesicles Myers, Grant A.; Harris, Joel M. Analytical Chemistry, Vol. 83, Issue 15 https://doi.org/10.1021/ac2012152	journal	August 2011
Confocal Raman Microscopy Probing of Temperature-Controlled Release from Individual, Optically-Trapped Phospholipid Vesicles Schaefer, Jonathan J.; Ma, Chaoxiong; Harris, Joel M. Analytical Chemistry, Vol. 84, Issue 21 https://doi.org/10.1021/ac302346n	journal	September 2012
Characterization of single levitated droplets by Raman spectroscopy Preston, Richard E.; Lettieri, Thomas R.; Semerjian, Hratch G. Langmuir, Vol. 1, Issue 3 https://doi.org/10.1021/la00063a018	journal	May 1985
Confocal micro-Raman spectroscopy of single biological cells using optical trapping and shifted excitation difference techniques Xie, Changan; Li, Yong-qing Journal of Applied Physics, Vol. 93, Issue 5 https://doi.org/10.1063/1.1542654	journal	March 2003
The Spectral Reflection Characteristics of a Smoked Magnesium Oxide Surface Gordon-Smith, G. W. Proceedings of the Physical Society. Section B, Vol. 65, Issue 4 https://doi.org/10.1088/0370-1301/65/4/305	journal	April 1952
Confocal Raman Microscopy of Optical-Trapped Particles in Liquids Cherney, Daniel P.; Harris, Joel M. Annual Review of Analytical Chemistry, Vol. 3, Issue 1 https://doi.org/10.1146/annurev-anchem-070109-103404	journal	June 2010
Structural resonances observed in the Raman spectra of optically levitated liquid droplets Thurn, Rudolf; Kiefer, Wolfgang Applied Optics, Vol. 24, Issue 10 https://doi.org/10.1364/AO.24.001515	journal	May 1985
Laser to single-mode fiber coupling in the laboratory Ladany, Ivan Applied Optics, Vol. 32, Issue 18 https://doi.org/10.1364/AO.32.003233	journal	June 1993
Observation of a single-beam gradient force optical trap for dielectric particles Ashkin, A.; Dziedzic, J. M.; Bjorkholm, J. E. Optics Letters, Vol. 11, Issue 5 https://doi.org/10.1364/OL.11.000288	journal	January 1986
Real-time Raman spectroscopy of optically trapped living cells and organelles Xie, Changan; Goodman, Charles; Dinno, Mumtaz A. Optics Express, Vol. 12, Issue 25 https://doi.org/10.1364/OPEX.12.006208	journal	January 2004
Modeling and Measuring the Effect of Refraction on the Depth Resolution of Confocal Raman Microscopy Everall, Neil J. Applied Spectroscopy, Vol. 54, Issue 6 https://doi.org/10.1366/0003702001950382	journal	June 2000
Temperature-Controlled Confocal Raman Microscopy to Detect Phase Transitions in Phospholipid Vesicles Fox, Christopher B.; Myers, Grant A.; Harris, Joel M. Applied Spectroscopy, Vol. 61, Issue 5 https://doi.org/10.1366/000370207780807786	journal	May 2007
Raman-Microsampling Technique Applying Optical Levitation by Radiation Pressure Thurn, R.; Kiefer, W. Applied Spectroscopy, Vol. 38, Issue 1 https://doi.org/10.1366/0003702844554440	journal	January 1984
Confocal Raman Microspectroscopy Using a Stigmatic Spectrograph and CCD Detector Williams, K. P. J.; Pitt, G. D.; Batchelder, D. N. Applied Spectroscopy, Vol. 48, Issue 2 https://doi.org/10.1366/0003702944028407	journal	February 1994
Nonlinear Scattering Effects in Optical Fibers Singh, Sunil Pratap; Gangwar, Ramgopal; Singh, Nar Progress In Electromagnetics Research, Vol. 74 https://doi.org/10.2528/PIER07051102	journal	January 2007
Calibration of reflectance standards Budde, W. Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry, Vol. 80A, Issue 4 https://doi.org/10.6028/jres.080A.057	journal	July 1976

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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diode lasers
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