Thermophilic biocatalysts for one-step conversion of citrus waste into lactic acid

Aulitto, Martina; Alfano, Alberto; Maresca, Emanuela; Avolio, Roberto; Errico, Maria Emanuela; Gentile, Gennaro; Cozzolino, Flora; Monti, Maria; Pirozzi, Annachiara; Donsì, Francesco; Cimini, Donatella; Schiraldi, Chiara; Contursi, Patrizia

doi:10.1007/s00253-023-12904-7

Thermophilic biocatalysts for one-step conversion of citrus waste into lactic acid

Journal Article · Sat Jan 20 00:00:00 EST 2024 · Applied Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s00253-023-12904-7· OSTI ID:2470649

Aulitto, Martina ^[1]; Alfano, Alberto ^[2]; Maresca, Emanuela ^[3]; Avolio, Roberto ^[4]; Errico, Maria Emanuela ^[4]; Gentile, Gennaro ^[4]; Cozzolino, Flora ^[3]; Monti, Maria ^[3]; Pirozzi, Annachiara ^[5]; Donsì, Francesco ^[5]; Cimini, Donatella ^[2]; Schiraldi, Chiara ^[2]; ^[6]

Univ. of Naples Federico II (Italy); National Research Council (CNR) (Italy); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
University of Campania L. Vanvitelli, Naples (Italy)
Univ. of Naples Federico II (Italy)
National Research Council (CNR) (Italy)
University of Salerno (Italy)
Univ. of Naples Federico II (Italy); National Biodiversity Future Center (NBFC) (Italy)

Agri-food residues offer significant potential as a raw material for the production of L-lactic acid through microbial fermentation. Weizmannia coagulans, previously known as Bacillus coagulans, is a spore-forming, lactic acid-producing, gram-positive, with known probiotic and prebiotic properties. This study aimed to evaluate the feasibility of utilizing untreated citrus waste as a sustainable feedstock for the production of L-lactic acid in a one-step process, by using the strain W. coagulans MA-13. By employing a thermophilic enzymatic cocktail (Cellic CTec2) in conjunction with the hydrolytic capabilities of MA-13, biomass degradation was enhanced by up to 62%. Moreover, batch and fed-batch fermentation experiments demonstrated the complete fermentation of glucose into L-lactic acid, achieving a concentration of up to 44.8 g/L. These results point to MA-13 as a microbial cell factory for one-step production of L-lactic acid, by combining cost-effective saccharification with MA-13 fermentative performance, on agri-food wastes. Moreover, the potential of this approach for sustainable valorization of agricultural waste streams is successfully proven.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470649

Journal Information:: Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology Journal Issue: 1 Vol. 108; ISSN 0175-7598

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (63)

PED interacts with Rac1 and regulates cell migration/invasion processes in human non‐small cell lung cancer cells Zanca, Ciro; Cozzolino, Flora; Quintavalle, Cristina Journal of Cellular Physiology, Vol. 225, Issue 1 https://doi.org/10.1002/jcp.22197	journal	July 2010
Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits Okano, Kenji; Tanaka, Tsutomu; Ogino, Chiaki Applied Microbiology and Biotechnology, Vol. 85, Issue 3 https://doi.org/10.1007/s00253-009-2280-5	journal	October 2009
Cross-linked natural IntegroPectin films from citrus biowaste with intrinsic antimicrobial activity Piacenza, Elena; Presentato, Alessandro; Alduina, Rosa Cellulose, Vol. 29, Issue 10 https://doi.org/10.1007/s10570-022-04627-1	journal	May 2022
Metagenomics of microbial and viral life in terrestrial geothermal environments Strazzulli, Andrea; Fusco, Salvatore; Cobucci-Ponzano, Beatrice Reviews in Environmental Science and Bio/Technology, Vol. 16, Issue 3 https://doi.org/10.1007/s11157-017-9435-0	journal	June 2017
RETRACTED ARTICLE: Recent advances in green technology and Industrial Revolution 4.0 for a sustainable future Bradu, Pragya; Biswas, Antara; Nair, Chandralekha Environmental Science and Pollution Research, Vol. 30, Issue 60 https://doi.org/10.1007/s11356-022-20024-4	journal	April 2022
Pretreatment and Lignocellulosic Chemistry Hu, Fan; Ragauskas, Art BioEnergy Research, Vol. 5, Issue 4 https://doi.org/10.1007/s12155-012-9208-0	journal	May 2012
Integral use of orange peel waste through the biorefinery concept: an experimental, technical, energy, and economic assessment Ortiz-Sanchez, M.; Solarte-Toro, J. C.; Orrego-Alzate, C. E. Biomass Conversion and Biorefinery, Vol. 11, Issue 2 https://doi.org/10.1007/s13399-020-00627-y	journal	February 2020
Effects of grinding processes on enzymatic degradation of wheat straw Silva, Gabriela Ghizzi D.; Couturier, Marie; Berrin, Jean-Guy Bioresource Technology, Vol. 103, Issue 1 https://doi.org/10.1016/J.BIORTECH.2011.09.073	journal	January 2012
The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials Sun, Shaoni; Sun, Shaolong; Cao, Xuefei Bioresource Technology, Vol. 199 https://doi.org/10.1016/J.BIORTECH.2015.08.061	journal	January 2016
Challenges and trends in membrane technology implementation for produced water treatment: A review Alzahrani, Salem; Mohammad, Abdul Wahab Journal of Water Process Engineering, Vol. 4 https://doi.org/10.1016/J.JWPE.2014.09.007	journal	December 2014
Factors affecting the fermentative lactic acid production from renewable resources Hofvendahl, Karin; Hahn–Hägerdal, Bärbel Enzyme and Microbial Technology, Vol. 26, Issue 2-4, p. 87-107 https://doi.org/10.1016/S0141-0229(99)00155-6	journal	February 2000
Current applications of citrus fruit processing waste: A scientific outlook Suri, Shweta; Singh, Anupama; Nema, Prabhat K. Applied Food Research, Vol. 2, Issue 1 https://doi.org/10.1016/j.afres.2022.100050	journal	June 2022
Simultaneous detoxification, saccharification and co-fermentation of oil palm empty fruit bunch hydrolysate for l-lactic acid production by Bacillus coagulans JI12 Ye, Lidan; Hudari, Mohammad Sufian Bin; Li, Zhi Biochemical Engineering Journal, Vol. 83 https://doi.org/10.1016/j.bej.2013.12.005	journal	February 2014
Insights from enzymatic degradation of cellulose and hemicellulose to fermentable sugars– a review Houfani, Aicha Asma; Anders, Nico; Spiess, Antje C. Biomass and Bioenergy, Vol. 134 https://doi.org/10.1016/j.biombioe.2020.105481	journal	March 2020
Fermentative utilization of coffee mucilage using Bacillus coagulans and investigation of down-stream processing of fermentation broth for optically pure l(+)-lactic acid production Neu, Anna-Katrin; Pleissner, Daniel; Mehlmann, Kerstin Bioresource Technology, Vol. 211 https://doi.org/10.1016/j.biortech.2016.03.122	journal	July 2016
New β-galactosidase producers with potential for prebiotic synthesis Silvério, Sara C.; Macedo, Eugénia A.; Teixeira, José A. Bioresource Technology, Vol. 250 https://doi.org/10.1016/j.biortech.2017.11.045	journal	February 2018
Bioconversion of biomass waste into high value chemicals Cho, Eun Jin; Trinh, Ly Thi Phi; Song, Younho Bioresource Technology, Vol. 298 https://doi.org/10.1016/j.biortech.2019.122386	journal	February 2020
Recent advances on the sustainable approaches for conversion and reutilization of food wastes to valuable bioproducts Ng, Hui Suan; Kee, Phei Er; Yim, Hip Seng Bioresource Technology, Vol. 302 https://doi.org/10.1016/j.biortech.2020.122889	journal	April 2020
One-pot process for lactic acid production from wheat straw by an adapted Bacillus coagulans and identification of genes related to hydrolysate-tolerance Ouyang, Shuiping; Zou, Lihua; Qiao, Hui Bioresource Technology, Vol. 315 https://doi.org/10.1016/j.biortech.2020.123855	journal	November 2020
In situ purification and enrichment of fructo-oligosaccharides by fermentative treatment with Bacillus coagulans and selective catalysis using immobilized fructosyltransferase Fan, Rong; Dresler, Josephine; Tissen, Dennis Bioresource Technology, Vol. 342 https://doi.org/10.1016/j.biortech.2021.125969	journal	December 2021
Recovery of carboxylic acids produced by fermentation López-Garzón, Camilo S.; Straathof, Adrie J. J. Biotechnology Advances, Vol. 32, Issue 5 https://doi.org/10.1016/j.biotechadv.2014.04.002	journal	September 2014
The extraction of bioactive compounds from food residues using microwaves Angiolillo, Luisa; Del Nobile, Matteo A.; Conte, Amalia Current Opinion in Food Science, Vol. 5 https://doi.org/10.1016/j.cofs.2015.10.001	journal	October 2015
A thermophilic enzymatic cocktail for galactomannans degradation Aulitto, Martina; Fusco, Francesca Anna; Fiorentino, Gabriella Enzyme and Microbial Technology, Vol. 111 https://doi.org/10.1016/j.enzmictec.2017.12.008	journal	April 2018
A review on the lactic acid fermentation from low-cost renewable materials: Recent developments and challenges Ahmad, Ashfaq; Banat, Fawzi; Taher, Hanifa Environmental Technology & Innovation, Vol. 20 https://doi.org/10.1016/j.eti.2020.101138	journal	November 2020
Extraction of phenolic compounds from dry and fermented orange pomace using supercritical CO2 and cosolvents Espinosa-Pardo, Faber Ariel; Nakajima, Vânia Mayumi; Macedo, Gabriela Alves Food and Bioproducts Processing, Vol. 101 https://doi.org/10.1016/j.fbp.2016.10.002	journal	January 2017
By-products from different citrus processes as a source of customized functional fibres Marín, Francisco R.; Soler-Rivas, Cristina; Benavente-García, Obdulio Food Chemistry, Vol. 100, Issue 2 https://doi.org/10.1016/j.foodchem.2005.04.040	journal	January 2007
Functionalization of pectin-depleted residue from different citrus by-products by high pressure homogenization Putri, Novita I.; Celus, Miete; Van Audenhove, Jelle Food Hydrocolloids, Vol. 129 https://doi.org/10.1016/j.foodhyd.2022.107638	journal	August 2022
Oil structuring through capillary suspensions prepared with wheat middlings micronized directly in oil by high-pressure homogenization Pirozzi, Annachiara; Posocco, Alfredo; Donsì, Francesco Food Hydrocolloids, Vol. 145 https://doi.org/10.1016/j.foodhyd.2023.109152	journal	December 2023
Extraction and recovery of bioactive soluble phenolic compounds from brocade orange (Citrus sinensis) peels: Effect of different extraction methods thereon Wang, Zhirong; Mei, Xiaofei; Chen, Xuhui LWT, Vol. 173 https://doi.org/10.1016/j.lwt.2022.114337	journal	January 2023
Converting citrus wastes into value-added products: Economic and environmently friendly approaches Sharma, Kavita; Mahato, Neelima; Cho, Moo Hwan Nutrition, Vol. 34 https://doi.org/10.1016/j.nut.2016.09.006	journal	February 2017
Simultaneous saccharification and fermentation of citrus peel waste by Saccharomyces cerevisiae to produce ethanol Wilkins, Mark R.; Widmer, Wilbur W.; Grohmann, Karel Process Biochemistry, Vol. 42, Issue 12 https://doi.org/10.1016/j.procbio.2007.09.006	journal	December 2007
Bio-refinery of orange peels waste: A new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin Boukroufa, Meryem; Boutekedjiret, Chahrazed; Petigny, Loïc Ultrasonics Sonochemistry, Vol. 24 https://doi.org/10.1016/j.ultsonch.2014.11.015	journal	May 2015
Valorisation of citrus processing waste: A review Zema, D. A.; Calabrò, P. S.; Folino, A. Waste Management, Vol. 80 https://doi.org/10.1016/j.wasman.2018.09.024	journal	October 2018
Submerged Citric Acid Fermentation on Orange Peel Autohydrolysate Rivas, Beatriz; Torrado, Ana; Torre, Paolo Journal of Agricultural and Food Chemistry, Vol. 56, Issue 7 https://doi.org/10.1021/jf073388r	journal	April 2008
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification Cox, Jürgen; Mann, Matthias Nature Biotechnology, Vol. 26, Issue 12 https://doi.org/10.1038/nbt.1511	journal	November 2008
A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities Ing, Nicole; Deng, Kai; Chen, Yan Scientific Reports, Vol. 11, Issue 1 https://doi.org/10.1038/s41598-021-91181-8	journal	June 2021
Intermolecular disulfide bond influences unphosphorylated STAT3 dimerization and function Butturini, Elena; Gotte, Giovanni; Dell'Orco, Daniele Biochemical Journal, Vol. 473, Issue 19 https://doi.org/10.1042/BCJ20160294	journal	September 2016
Draft Genome Sequence of Bacillus coagulans MA-13, a Thermophilic Lactic Acid Producer from Lignocellulose Aulitto, Martina; Fusco, Salvatore; Franzén, Carl Johan Microbiology Resource Announcements, Vol. 8, Issue 23 https://doi.org/10.1128/mra.00341-19	journal	June 2019
Production of lactic acid from pasta wastes using a biorefinery approach Marzo-Gago, Cristina; Venus, Joachim; López-Gómez, José Pablo Biotechnology for Biofuels and Bioproducts, Vol. 15, Issue 1 https://doi.org/10.1186/S13068-022-02222-X	journal	November 2022
The identification of a novel Sulfolobus islandicus CAMP-like peptide points to archaeal microorganisms as cell factories for the production of antimicrobial molecules Notomista, Eugenio; Falanga, Annarita; Fusco, Salvatore Microbial Cell Factories, Vol. 14, Issue 1 https://doi.org/10.1186/s12934-015-0302-9	journal	September 2015
Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando Microbial Cell Factories, Vol. 20, Issue 1 https://doi.org/10.1186/s12934-021-01553-y	journal	March 2021
Bacillus coagulans MA-13: a promising thermophilic and cellulolytic strain for the production of lactic acid from lignocellulosic hydrolysate Aulitto, Martina; Fusco, Salvatore; Bartolucci, Simonetta Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0896-8	journal	September 2017
Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation Aulitto, Martina; Fusco, Salvatore; Nickel, David Benjamin Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1382-2	journal	February 2019
Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction Tom, Lauren M.; Aulitto, Martina; Wu, Yu-Wei Microbiome, Vol. 10, Issue 1 https://doi.org/10.1186/s40168-022-01377-x	journal	October 2022
Microorganisms from harsh and extreme environments: a collection of living strains at ACUF (Naples, Italy) D’Elia, Luigi; Del Mondo, Angelo; Santoro, Mariano Ecological Questions, Vol. 29, Issue 3 https://doi.org/10.12775/EQ.2018.023	journal	July 2018
Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry Wang, Yaqi; Wu, Jiangtao; Lv, Mengxin Frontiers in Bioengineering and Biotechnology, Vol. 9 https://doi.org/10.3389/fbioe.2021.612285	journal	May 2021
Fermentative Lactic Acid Production From Lignocellulosic Feedstocks: From Source to Purified Product Yankov, Dragomir Frontiers in Chemistry, Vol. 10 https://doi.org/10.3389/fchem.2022.823005	journal	March 2022
Indexing energy and cost of the pretreatment for economically efficient bioenergy generation No authors listed Frontiers in Energy Research, Vol. 10 https://doi.org/10.3389/fenrg.2022.1060599	journal	January 2023
Evaluation of the antimicrobial effects of Capsicum, Nigella sativa, Musa paradisiaca L., and Citrus limetta: A review Bhatti, Sonia Abid; Hussain, Muhammad Hammad; Mohsin, Muhammad Zubair Frontiers in Sustainable Food Systems, Vol. 6 https://doi.org/10.3389/fsufs.2022.1043823	journal	December 2022
Application of Bacillus coagulans in Animal Husbandry and Its Underlying Mechanisms Zhou, Yuanhao; Zeng, Zihan; Xu, Yibin Animals, Vol. 10, Issue 3 https://doi.org/10.3390/ani10030454	journal	March 2020
Production of D-Lactic Acid by the Fermentation of Orange Peel Waste Hydrolysate by Lactic Acid Bacteria Bustamante, Daniel; Tortajada, Marta; Ramón, Daniel Fermentation, Vol. 6, Issue 1 https://doi.org/10.3390/fermentation6010001	journal	December 2019
Chestnut Shells as Waste Material for Succinic Acid Production from Actinobacillus succinogenes 130Z Ventrone, Michela; Schiraldi, Chiara; Squillaci, Giuseppe Fermentation, Vol. 6, Issue 4 https://doi.org/10.3390/fermentation6040105	journal	November 2020
Cellulose Isolation from Tomato Pomace Pretreated by High-Pressure Homogenization Pirozzi, Annachiara; Ferrari, Giovanna; Donsì, Francesco Foods, Vol. 11, Issue 3 https://doi.org/10.3390/foods11030266	journal	January 2022
A Comparative Analysis of Weizmannia coagulans Genomes Unravels the Genetic Potential for Biotechnological Applications Aulitto, Martina; Martinez-Alvarez, Laura; Fiorentino, Gabriella International Journal of Molecular Sciences, Vol. 23, Issue 6 https://doi.org/10.3390/ijms23063135	journal	March 2022
Alicyclobacillus mali FL18 as a Novel Source of Glycosyl Hydrolases: Characterization of a New Thermophilic β-Xylosidase Tolerant to Monosaccharides Salzano, Flora; Aulitto, Martina; Fiorentino, Gabriella International Journal of Molecular Sciences, Vol. 23, Issue 22 https://doi.org/10.3390/ijms232214310	journal	November 2022
Insight into CAZymes of Alicyclobacillus mali FL18: Characterization of a New Multifunctional GH9 Enzyme Carbonaro, Miriam; Aulitto, Martina; Gallo, Giovanni International Journal of Molecular Sciences, Vol. 24, Issue 1 https://doi.org/10.3390/ijms24010243	journal	December 2022
Citrus Pomace Biomass as a Source of Pectin and Lignocellulose Fibers: From Waste to Upgraded Biocomposites for Mulching Applications Zannini, Domenico; Dal Poggetto, Giovanni; Malinconico, Mario Polymers, Vol. 13, Issue 8 https://doi.org/10.3390/polym13081280	journal	April 2021
Biotransformation of Citrus Waste-I: Production of Biofuel and Valuable Compounds by Fermentation Mahato, Neelima; Sharma, Kavita; Sinha, Mukty Processes, Vol. 9, Issue 2 https://doi.org/10.3390/pr9020220	journal	January 2021
The identification of a novel Sulfolobus islandicus CAMP-like peptide points to archaeal microorganisms as cell factories for the production of antimicrobial molecules Notomista, Eugenio; Falanga, Annarita; Fusco, Salvatore Figshare https://doi.org/10.6084/m9.figshare.c.3646283	collection	January 2015
Bacillus coagulans MA-13: a promising thermophilic and cellulolytic strain for the production of lactic acid from lignocellulosic hydrolysate Aulitto, Martina; Fusco, Salvatore; Bartolucci, Simonetta Figshare https://doi.org/10.6084/m9.figshare.c.3874402.v1	collection	January 2017
Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation Aulitto, Martina; Fusco, Salvatore; Nickel, David Figshare https://doi.org/10.6084/m9.figshare.c.4419416.v1	collection	January 2019
Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando figshare https://doi.org/10.6084/m9.figshare.c.5345105	collection	January 2021
Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction Tom, Lauren M.; Aulitto, Martina; Wu, Yu-Wei figshare https://doi.org/10.6084/m9.figshare.c.6264237.v1	collection	January 2022

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
W. coagulans
citrus waste
fed-batch fermentation
lactic acid

Thermophilic biocatalysts for one-step conversion of citrus waste into lactic acid

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References (63)

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