Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation

Aulitto, Martina; Fusco, Salvatore; Nickel, David Benjamin; Bartolucci, Simonetta; Contursi, Patrizia; Franzén, Carl Johan

doi:10.1186/s13068-019-1382-2

Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation

Journal Article · Thu Feb 28 00:00:00 EST 2019 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-019-1382-2· OSTI ID:1581059

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Univ. of Naples Federico II (Italy); Chalmers Univ. Technology, Gothenburg (Sweden)
Chalmers Univ. Technology, Gothenburg (Sweden)
Univ. of Naples Federico II (Italy)

Background: Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect microbial growth and metabolism are released from the biomass upon thermochemical pre-treatment. So far, susceptibility of bacterial strains to biomass-derived inhibitors still represents a major barrier to lactic acid production from lignocellulose. Detoxification of the pre-treated lignocellulosic material by water washing is commonly performed to alleviate growth inhibition of the production microorganism and achieve higher production rates. Results: In this study, we assessed the feasibility of replacing the washing step with integrated cellular adaptation during pre-culture of Bacillus coagulans MA-13 prior to simultaneous saccharification and lactic acid fermentation of steam exploded wheat straw. Using a seed culture pre-exposed to 30% hydrolysate led to 50% shorter process time, 50% higher average volumetric and 115% higher average specific productivity than when using cells from a hydrolysate-free seed culture. Conclusions: Pre-exposure of B. coagulans MA-13 to hydrolysate supports adaptation to the actual production medium. This strategy leads to lower process water requirements and combines cost-effective seed cultivation with physiological pre-adaptation of the production strain, resulting in reduced lactic acid production costs.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1581059

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 12; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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MOESM1 of Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation Aulitto, Martina; Fusco, Salvatore; Nickel, David https://doi.org/10.6084/m9.figshare.7790840 The current record is supplemented by this dataset	dataset	January 2019
MOESM1 of Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation Aulitto, Martina; Fusco, Salvatore; Nickel, David https://doi.org/10.6084/m9.figshare.7790840.v1 The current record is supplemented by this dataset	dataset	January 2019
Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation Aulitto, Martina; Fusco, Salvatore; Nickel, David https://doi.org/10.6084/m9.figshare.c.4419416 The current record is supplemented by this collection	collection	January 2019
Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation Aulitto, Martina; Fusco, Salvatore; Nickel, David https://doi.org/10.6084/m9.figshare.c.4419416.v1 The current record is supplemented by this collection	collection	January 2019

Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation

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