Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando; Cozzolino, Flora; Monti, Maria; Moracci, Marco; Fiorentino, Gabriella; Limauro, Danila; Bartolucci, Simonetta; Contursi, Patrizia

doi:10.1186/s12934-021-01553-y

Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

Journal Article · Thu Mar 18 00:00:00 EDT 2021 · Microbial Cell Factories

DOI:https://doi.org/10.1186/s12934-021-01553-y· OSTI ID:1815946

Aulitto, Martina ^[1]; Strazzulli, Andrea ^[2]; Sansone, Ferdinando ^[3]; Cozzolino, Flora ^[4]; Monti, Maria ^[4]; Moracci, Marco ^[5]; Fiorentino, Gabriella ^[6]; Limauro, Danila ^[7]; Bartolucci, Simonetta ^[3]; ^[8]

Univ. of Naples Federico II (Italy). Dept. of Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Division of Biological Systems and Engineering; OSTI
Univ. of Naples Federico II (Italy). Dept. of Biology; Univ. of Naples Federico II (Italy). Task Force on Microbiome Studies
Univ. of Naples Federico II (Italy). Dept. of Biology
Univ. of Naples Federico II (Italy). Dept. of Chemical Sciences; Univ. of Naples Federico II (Italy). CEINGE Advanced Biotechnologies
Univ. of Naples Federico II (Italy). Dept. of Biology; Univ. of Naples Federico II (Italy). Task Force on Microbiome Studies; Italian National Research Council (INRC), Naples (Italy). Inst. of Biosciences and BioResources
Univ. of Naples Federico II (Italy). Dept. of Biology; Univ. of Naples Federico II (Italy). BAT Center—Interuniversity Center for Studies On Bioinspired Agro-Environmental Technology
Univ. of Naples Federico II (Italy). Dept. of Biology; Univ. of Naples Federico II (Italy). BAT Center—Interuniversity Center for Studies On Bioinspired Agro-Environmental Technology
Univ. of Naples Federico II (Italy). Dept. of Biology; Univ. of Naples Federico II (Italy). Task Force on Microbiome Studies; Univ. of Naples Federico II (Italy). BAT Center—Interuniversity Center for Studies On Bioinspired Agro-Environmental Technology

The spore-forming lactic acid bacterium Bacillus coagulans MA-13 has been isolated from canned beans manufacturing and successfully employed for the sustainable production of lactic acid from lignocellulosic biomass. Among lactic acid bacteria, B. coagulans strains are generally recognized as safe (GRAS) for human consumption. Low-cost microbial production of industrially valuable products such as lactic acid and various enzymes devoted to the hydrolysis of oligosaccharides and lactose, is of great importance to the food industry. Specifically, α- and β-galactosidases are attractive for their ability to hydrolyze not-digestible galactosides present in the food matrix as well as in the human gastrointestinal tract. In this work we have explored the potential of B. coagulans MA-13 as a source of metabolites and enzymes to improve the digestibility and the nutritional value of food. A combination of mass spectrometry analysis with conventional biochemical approaches has been employed to unveil the intra- and extra- cellular glycosyl hydrolase (GH) repertoire of B. coagulans MA-13 under diverse growth conditions. The highest enzymatic activity was detected on β-1,4 and α-1,6-glycosidic linkages and the enzymes responsible for these activities were unambiguously identified as β-galactosidase (GH42) and α-galactosidase (GH36), respectively. Whilst the former has been found only in the cytosol, the latter is localized also extracellularly. The export of this enzyme may occur through a not yet identified secretion mechanism, since a typical signal peptide is missing in the α-galactosidase sequence. A full biochemical characterization of the recombinant β-galactosidase has been carried out and the ability of this enzyme to perform homo- and hetero-condensation reactions to produce galacto-oligosaccharides, has been demonstrated. Probiotics which are safe for human use and are capable of producing high levels of both α-galactosidase and β-galactosidase are of great importance to the food industry. In this work we have proven the ability of B. coagulans MA-13 to over-produce these two enzymes thus paving the way for its potential use in treatment of gastrointestinal diseases.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1815946

Journal Information:: Microbial Cell Factories, Journal Name: Microbial Cell Factories Journal Issue: 1 Vol. 20; ISSN 1475-2859

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando figshare https://doi.org/10.6084/m9.figshare.c.5345105	collection	January 2021

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59 BASIC BIOLOGICAL SCIENCES
Bacillus coagulans
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Thermophilic
Transgalactosylation
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β-galactosidase

Additional file 1 of Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.14247569 The current record is supplemented by this dataset	dataset	January 2021
Additional file 1 of Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.14247569.v1 The current record is supplemented by this dataset	dataset	January 2021
Additional file 2 of Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.14247575 The current record is supplemented by this audiovisual	audiovisual	January 2021
Additional file 2 of Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.14247575.v1 The current record is supplemented by this audiovisual	audiovisual	January 2021
Additional file 3 of Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.14247578 The current record is supplemented by this audiovisual	audiovisual	January 2021
Additional file 3 of Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.14247578.v1 The current record is supplemented by this audiovisual	audiovisual	January 2021
Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.c.5345105 The current record is supplemented by this collection	collection	January 2021
Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase Aulitto, Martina; Strazzulli, Andrea; Sansone, Ferdinando https://doi.org/10.6084/m9.figshare.c.5345105.v1 The current record is supplemented by this collection	collection	January 2021

Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

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