Structural characterization of high-protein system through ultra-small and small-angle X-ray scattering

Chen, Da; Ilavsky, Jan

doi:10.1016/j.cofs.2024.101204

Structural characterization of high-protein system through ultra-small and small-angle X-ray scattering

Journal Article · Sun Aug 04 00:00:00 EDT 2024 · Current Opinion in Food Science

DOI:https://doi.org/10.1016/j.cofs.2024.101204· OSTI ID:2433818

^[1]; Ilavsky, Jan ^[2]

Purdue Univ., West Lafayette, IN (United States)
Argonne National Laboratory (ANL), Argonne, IL (United States)

High-protein systems exhibit a hierarchical structure consisting of interconnected multiscale length assemblies. Recent investigations are directed toward identifying these structural units under various processing and environmental conditions to establish structure–function correlations. Ultra-small and small-angle X-ray scattering (USAXS/SAXS) has become a crucial tool for characterizing the structures of proteins and their clusters/aggregates, ranging from nanometers to micrometers, with minimal disruption to their original state. Here, this review first describes the facilities and principles of X-ray scattering, followed by discussions on the analysis of scattering data, including the interpretation of fitting models. It then delves into the main applications of USAXS/SAXS in plant and dairy protein-rich systems. Future strategies to enhance the utilization of scattering techniques for elucidating the structure of high-protein systems are also included.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2433818

Journal Information:: Current Opinion in Food Science, Journal Name: Current Opinion in Food Science Vol. 59; ISSN 2214-7993

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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