Characterization of zein assemblies by ultra-small-angle X-ray scattering

Uzun, Suzan; Ilavsky, Jan; Padua, Graciela Wild

doi:10.1039/c6sm02717b

Characterization of zein assemblies by ultra-small-angle X-ray scattering

Journal Article · Thu Mar 23 00:00:00 EDT 2017 · Soft Matter

DOI:https://doi.org/10.1039/c6sm02717b· OSTI ID:1372296

Uzun, Suzan; Ilavsky, Jan; Padua, Graciela Wild

Zein, a protein of corn, has an amphiphilic molecule capable of self-assembly into distinctly different structures. In this work, ultra-small-angle X-ray scattering (USAXS) was applied to investigate the formation of self-assembled zein structures in binary solvent systems of ethanol and water. Our study included observing structural changes due to aging. Three hierarchical structures were identified in zein-solvent systems, molecular zein 2D films, believed to be formed by zein rods assembled first into one-dimensional fibers and then into two-dimensional films, and 3D spherical aggregates. Aging did not change the size or shape of primary units, but promoted their self-assembly into intermediate 2D structures and shaped 3D structures into well19 defined spheres. We found that the rheological parameters, consistency index (K) and behavior index (n), storage and loss moduli (G’ and G”) were also measured. K and n, changed markedly with aging, from nearly Newtonian low consistency fresh samples to highly viscous pseudoplastic aged samples. G’ and G” increased with aging for all samples reflecting increased interactions among zein self-assembled structures. Furthermore, viscoelastic parameters indicated that zein dispersions formed gels upon aging. It was observed that USAX reported on molecular scale self-assembly processes, while rheological measurements reported on the macroscale interaction between self-assembled particles. Raman spectra suggested that α-helix to β-sheet transformations prompted zein self-assembly, which influenced the size and morphology of molecular assemblies and ultimately the rheological properties of zein dispersions.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); U.S. Department of Agriculture (USDA) - National Institute of Food and Agriculture (NIFA)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1372296

Journal Information:: Soft Matter, Journal Name: Soft Matter Journal Issue: 16 Vol. 13; ISSN SMOABF; ISSN 1744-683X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

36 MATERIALS SCIENCE
USAXS
aging
hierarchical nanostructures
self-assembly
zein

Characterization of zein assemblies by ultra-small-angle X-ray scattering

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