Galactomannan utilization by Cellvibrio japonicus relies on a single essential α‐galactosidase encoded by the aga27A gene

Novak, Jessica K.; Gardner, Jeffrey G.

doi:10.1111/mmi.15024

Galactomannan utilization by Cellvibrio japonicus relies on a single essential α‐galactosidase encoded by the aga27A gene

Journal Article · Wed Jan 04 23:00:00 EST 2023 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.15024· OSTI ID:2420329

Novak, Jessica K. ^[1]; ^[2]

Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States); OSTI
Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)

Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions. Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose. One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has 10 predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27. Here we describe a systems biology approach to identify and characterize the essential mannan-degrading components in this bacterium. The transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode carbohydrate active enzymes (CAZymes) when mannan was actively being degraded. A comprehensive mutational analysis characterized 54 CAZyme-encoding genes in the context of mannan utilization. Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential. Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0014183

OSTI ID:: 2420329

Alternate ID(s):: OSTI ID: 1914357

Journal Information:: Molecular Microbiology, Journal Name: Molecular Microbiology Journal Issue: 3 Vol. 119; ISSN 0950-382X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Biochemistry & Molecular Biology
Cellvibrio japonicus
Microbiology
alpha-galactosidase
carbohydrate-active enzyme
galacto
mannan
polysaccharide degradation

Galactomannan utilization by Cellvibrio japonicus relies on a single essential α‐galactosidase encoded by the aga27A gene

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