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The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization

Journal Article · · Molecular Microbiology
DOI:https://doi.org/10.1111/mmi.13903· OSTI ID:1415270
 [1];  [2];  [2];  [2];  [2]
  1. Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States). Dept. of Biological Sciences; University of Maryland - Baltimore County
  2. Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States). Dept. of Biological Sciences
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Lignocellulose degradation by microbes plays a central role in global carbon cycling, human gut metabolism, and renewable energy technologies. While considerable effort has been put into understanding the biochemical aspects of lignocellulose degradation, much less work has been done to understand how these enzymes work in an in vivo context. Here in this paper, we report a systems level study of xylan degradation in the saprophytic bacterium Cellvibrio japonicus. Transcriptome analysis indicated seven genes that encode carbohydrate active enzymes were up-regulated during growth with xylan containing media. In-frame deletion analysis of these genes found that only gly43F is critical for utilization of xylo-oligosaccharides, xylan, and arabinoxylan. Heterologous expression of gly43F was sufficient for the utilization of xylo-oligosaccharides in Escherichia coli. Additional analysis found that the xyn11A, xyn11B, abf43L, abf43K, and abf51A gene products were critical for utilization of arabinoxylan. Furthermore, a predicted transporter (CJA_1315) was required for effective utilization of xylan substrates, and we propose this unannotated gene be called xntA (xylan transporter A). Our major findings are 1) C. japonicus employs both secreted and surface associated enzymes for xylan degradation, which differs from the strategy used for cellulose degradation, and 2) a single cytoplasmic β-xylosidase is essential for the utilization of xylo-oligosaccharides.

Research Organization:
Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division
Grant/Contract Number:
SC0014183
OSTI ID:
1415270
Alternate ID(s):
OSTI ID: 1416401
Journal Information:
Molecular Microbiology, Journal Name: Molecular Microbiology Journal Issue: 5 Vol. 107; ISSN 0950-382X
Publisher:
WileyCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (4)

High-throughput screening of environmental polysaccharide-degrading bacteria using biomass containment and complex insoluble substrates journal February 2020
Systems analysis of the glycoside hydrolase family 18 enzymes from Cellvibrio japonicus characterizes essential chitin degradation functions journal January 2018
Two forms of phosphomannomutase in gammaproteobacteria: The overlooked membrane‐bound form of AlgC is required for twitching motility of Lysobacter enzymogenes journal May 2019
Complete Genome Sequences of Cellvibrio japonicus Strains with Improved Growth When Using α-Diglucosides journal October 2019

Figures / Tables (7)

Fig. 1. C. japonicus CAZyme genes up-regulated during exponential growth using xylan.(p. 30)figure Fig. 1. C. japonicus CAZyme genes up-regulated during exponential growth using xylan.
Fig. 2. Secreted CAZymes are required for arabinoxylan utilization in C. japonicus.(p. 31)figure Fig. 2. Secreted CAZymes are required for arabinoxylan utilization in C. japonicus.
Fig. 3. The Xyn11A and Xyn11B endo-xylanases are required for arabinoxylan utilization in C. japonicus.(p. 33)figure Fig. 3. The Xyn11A and Xyn11B endo-xylanases are required for arabinoxylan utilization in C. japonicus.
Fig. 4.(p. 35)figure Fig. 4.
Fig. 5.(p. 37)figure Fig. 5.
Fig. 6.(p. 39)figure Fig. 6.
Fig. 7.(p. 40)figure Fig. 7.

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Cellvibrio japonicus
polysaccharide degradation
xylan
xylanase
β-xylosidase