Efficient chito–oligosaccharide utilization requires two TonB–dependent transporters and one hexosaminidase in Cellvibrio japonicus

Monge, Estela C.; Gardner, Jeffrey G.

doi:10.1111/mmi.14717

Title: Efficient chito–oligosaccharide utilization requires two TonB–dependent transporters and one hexosaminidase in Cellvibrio japonicus

Journal Article · Thu Mar 18 00:00:00 EDT 2021 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.14717· OSTI ID:1774542

Monge, Estela C. ^[1];

^[1]

Univ. of Maryland - Baltimore County, MD (United States)

Abstract Chitin utilization by microbes plays a significant role in biosphere carbon and nitrogen cycling, and studying the microbial approaches used to degrade chitin will facilitate our understanding of bacterial strategies to degrade a broad range of recalcitrant polysaccharides. The early stages of chitin depolymerization by the bacterium Cellvibrio japonicus have been characterized and are dependent on one chitin‐specific lytic polysaccharide monooxygenase and nonredundant glycoside hydrolases from the family GH18 to generate chito‐oligosaccharides for entry into metabolism. Here, we describe the mechanisms for the latter stages of chitin utilization by C. japonicus with an emphasis on the fate of chito‐oligosaccharides. Our systems biology approach combined transcriptomics and bacterial genetics using ecologically relevant substrates to determine the essential mechanisms for chito‐oligosaccharide transport and catabolism in C. japonicus . Using RNAseq analysis we found a coordinated expression of genes that encode polysaccharide‐degrading enzymes. Mutational analysis determined that the hex20B gene product, predicted to encode a hexosaminidase, was required for efficient utilization of chito‐oligosaccharides. Furthermore, two gene loci (CJA_0353 and CJA_1157), which encode putative TonB‐dependent transporters, were also essential for chito‐oligosaccharides utilization. This study further develops our model of C. japonicus chitin metabolism and may be predictive for other environmentally or industrially important bacteria.

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Research Organization:: Univ. of Maryland - Baltimore County, MD (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0014183

OSTI ID:: 1774542

Alternate ID(s):: OSTI ID: 1880402

Journal Information:: Molecular Microbiology, Vol. 116, Issue 2; ISSN 0950-382X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Cellvibrio japonicus
chitin
glycoside hydrolase
hexosaminidase
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Title: Efficient chito–oligosaccharide utilization requires two TonB–dependent transporters and one hexosaminidase in Cellvibrio japonicus

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References (83)

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