Crystal structure of Campylobacter jejuni lipoprotein Cj1090c

Paek, Seonghee; Kawai, Fumihiro; Venisetty, Nilamani; Kim, Youngchang; Yeo, Hye‐Jeong

doi:10.1002/prot.26434

Crystal structure of Campylobacter jejuni lipoprotein Cj1090c

Journal Article · Thu Oct 06 00:00:00 EDT 2022 · Proteins

DOI:https://doi.org/10.1002/prot.26434· OSTI ID:2378032

Paek, Seonghee ^[1]; Kawai, Fumihiro ^[1]; Venisetty, Nilamani ^[1]; Kim, Youngchang ^[2]; ^[1]

Univ. of Houston, TX (United States)
Argonne National Laboratory (ANL), Argonne, IL (United States). Structural Biology Center

In Gram-negative bacteria, lipopolysaccharide (LPS) is an essential component of the asymmetric outer membrane (OM). LptE is an OM lipoprotein that forms a complex with the β-barrel OM protein, LptD. Incorporation of LPS into the OM outer leaflet is essential for bacterial viability, and mediated by the LptD/E complex. The genome of Campylobacter jejuni, a major foodborne pathogen, contains over 20 putative lipoproteins including Cj1090c. Here, we report the crystal structure of Cj1090c at 2.4 Å resolution, revealing structural evidence for LptE in C. jejuni. The analysis of this crystal structure, along with the genomic context, allows us to propose the C. jejuni LPS transport system for the first time, and also permits for discussion of the features of the LptD/E complex of C. jejuni.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; Welch Foundation

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2378032

Journal Information:: Proteins, Journal Name: Proteins Journal Issue: 3 Vol. 91; ISSN 0887-3585

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Campylobacter jejuni
Cj1090c
LptE
crystal structure
lipoprotein

Crystal structure of Campylobacter jejuni lipoprotein Cj1090c

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References (31)

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