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Structural Basis for the Lipopolysaccharide Export Activity of the Bacterial Lipopolysaccharide Transport System

Journal Article · · International Journal of Molecular Sciences (Online)
DOI:https://doi.org/10.3390/ijms19092680· OSTI ID:1600798
 [1];  [1]
  1. Queen's Univ., Kingston, ON (Canada)
+ Show Author Affiliations
Gram-negative bacteria have a dense outer membrane (OM) coating of lipopolysaccharides, which is essential to their survival. This coating is assembled by the LPS (lipopolysaccharide) transport (Lpt) system, a coordinated seven-subunit protein complex that spans the cellular envelope. LPS transport is driven by an ATPase-dependent mechanism dubbed the “PEZ” model, whereby a continuous stream of LPS molecules is pushed from subunit to subunit. This review explores recent structural and functional findings that have elucidated the subunit-scale mechanisms of LPS transport, including the novel ABC-like mechanism of the LptB2FG subcomplex and the lateral insertion of LPS into the OM by LptD/E. New questions are also raised about the functional significance of LptA oligomerization and LptC. The tightly regulated interactions between these connected subcomplexes suggest a pathway that can react dynamically to membrane stress and may prove to be a valuable target for new antibiotic therapies for Gram-negative pathogens.
Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
Natural Sciences and Engineering Research Council of Canada (NSERC)
OSTI ID:
1600798
Journal Information:
International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 9 Vol. 19; ISSN 1422-0067
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
ENGLISH

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Structural features underlying recognition and translocation of extracellular polysaccharides journal February 2019
Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects journal January 2019

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Gram-negative bacteria
lipopolysaccharides
membrane transport