Zika virus infected primary microglia impairs NPCs proliferation and differentiation

Journal Article · Thu Mar 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.02.118· OSTI ID:23137305

Wang, Jin; Liu, Jing; Zhou, Rui; Ding, Xin; Zhang, Qipeng; Zhang, Chenyu; Li, Liang ^[1]

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1. State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210046 (China)

Highlights: • ZIKV infects primary microglia and induce inflammatory response. • ZIKV infected microglia decreases NPCs proliferation. • ZIKV infected microglia promote astrogenesis at the expense of neurogenesis. • Cytokines secreted by ZIKV infected microglia decrease neurogenesis. Zika virus (ZIKV) can lead to severe birth defects especially microcephaly in newborns by infecting human neural progenitors and impairing brain development. However, as the resident immune cells in the brain, the role of microglia in the ZIKV pathology is not clearly defined. To understand the interplay between immune response and neural cells, we investigate the interaction between microglia and NPCs during ZIKV infection. Our results demonstrate that primary microglia infected with ZIKV induces an inflammatory response similar to that in human, producing high level of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 1β (IL-1β) and inducible nitric oxide synthase (iNOS). Furthermore, conditional medium (CM) of ZIKV infected microglia showed inhibitory effects on cell proliferation and neuronal differentiation of neural precursor cells (NPCs) derived from E14 mice brain. Blocking cytokines in the CM remarkably improved neurogenesis and decreased astrocytic differentiation of NPCs. Together, our results suggest that microglia mediated neuroinflammation plays an important role in neuropathogenesis during ZIKV infection.

• OSTI ID: 23137305
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 497; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BRAIN
CELL PROLIFERATION
INFLAMMATION
LYMPHOKINES
MICE
NEONATES
NITRIC OXIDE
ZIKA VIRUS