Upregulation of circ-UBAP2 predicts poor prognosis and promotes triple-negative breast cancer progression through the miR-661/MTA1 pathway

Journal Article · Thu Nov 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.10.026· OSTI ID:23134101

Wang, Shengting; Li, Qian; Wang, Yufang; Li, Xiaoming; Wang, Rui; Kang, Yuhua ^[1]; Xue, Xukai ^[2]; Meng, Rui; Wei, Qi; Feng, Xinghua ^[1]

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1. Department of Clinical Medicine, Peihua University, Xi'an, 710125 (China)
2. Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, 710054 (China)

Highlights: • circ-UBAP2 is significantly up-regulated in TNBC and predicts poor prognosis. • Knockdown of circ-UBAP2 remarkably inhibits TNBC progression both in vitro and in vivo. • circ-UBAP2 exerts its oncogenic effect via miR-661/MTA1 axis in TNBC. Recently, a large number of studies have shown that circular RNA (circRNA) is involved in the development and progression of human cancer. However, its role in triple-negative breast cancer (TNBC) remains largely unknown. In this study, a novel circRNA, circ-UBAP2 (hsa{sub c}irc{sub 0}001846), was shown to be markedly upregulated in TNBC. Moreover, high circ-UBAP2 expression was closely associated with larger tumour size (p = 0.003), advanced TNM stage (p = 0.005), lymph node metastasis (p = 0.002), and unfavourable prognosis (p = 0.0256). Functionally, lentivirus-mediated stable knockdown of circ-UBAP2 dramatically weakened the ability of TNBC cells to proliferate and migrate and induced apoptosis in vitro and in vivo. Regarding the mechanism, we found that circ-UBAP2 was mainly observed in the cytoplasm and was capable of sponging miRNA-661 to increase the expression of the oncogene MTA1. Additionally, silencing of miRNA-661 or overexpression of MTA1 could partially rescue the attenuated malignant phenotype caused by circ-UBAP2 knockdown. Taken together, our data reveal that circ-UBAP2 plays a vital regulatory role in TNBC via the miR-661/MTA1 axis and may serve as a promising therapeutic target for TNBC patients.

• OSTI ID: 23134101
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 505; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
CYTOPLASM
LYMPH NODES
MAMMARY GLANDS
METASTASES
NEOPLASMS
ONCOGENES
RNA