Circular RNA hsa-circ-0010729 regulates vascular endothelial cell proliferation and apoptosis by targeting the miR-186/HIF-1α axis

Journal Article · Sat Aug 19 04:00:00 EDT 2017 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.05.164· OSTI ID:22719029

Dang, Rui-Ying ^[1]; Liu, Feng-Li ^[2]; Li, Yan ^[1]

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1. Affiliated Hospital of Hebei Engineering University, Handan, Hebei Province, 056038 (China)
2. Medical College of Hebei University of Engineering, Handan, Hebei Province, 056038 (China)

Circular RNAs (circRNAs) are a group of non-protein-coding RNAs generated from back splicing. Emerging evidence has demonstrated its vital regulation on angiogenesis. However, the underlying mechanism responsible for circRNAs effects on vascular endothelial cells is still unclear. In the present study, we screened the expression profiles and investigated the physiological role of circRNAs in hypoxia-induced human umbilical vein endothelial cells (HUVECs). Using circRNA microarray analysis, we identified 36 circRNAs that were significantly dysregulated including 14 down-regulated circRNAs and 22 up-regulated with 2-fold change (P < 0.05). From the over-expressed circRNAs, hsa-circ-0010729 was selected as candidate circRNA and which was validated to be significantly up-regulated using RT-PCR. In loss-of-function experiments of HUVECs, hsa-circ-0010729 knockdown suppressed the proliferation and migration ability and enhanced apoptosis. Bioinformatic prediction and luciferase assay revealed that hsa-circ-0010729 and hypoxia inducible factor 1 alpha (HIF-1α) were targeted by miR-186. Validation experiments verified that hsa-circ-0010729 was co-expressed with HIF-1α, being negatively correlated with miR-186. Moreover, rescue experiments demonstrated that miR-186 inhibitor could reverse the role of hsa-circ-0010729 knockdown on HUVECs progression. Overall, the present study identifies the crucial regulation of hsa-circ-0010729 on vascular endothelial cell proliferation and apoptosis via targeting miR-186/HIF-1α axis. - Highlights: • Circular RNA expression profile identifies hsa-circ-0010729 is up-regulated in hypoxia-induced HUVECs. • Hsa-circ-0010729 knockdown suppresses the proliferation and migration ability, and enhanced the apoptosis. • Hsa-circ-0010729 acts as an endogenous sponge of miR-186. • HIF-1α is targeted by miR-186. • Hsa-circ-0010729 regulates vascular endothelial cells proliferation and apoptosis via targeting miR-186/HIF-1α axis.

• OSTI ID: 22719029
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 490; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALBUMINS
ANOXIA
APOPTOSIS
BLOOD SERUM
CELL PROLIFERATION
LUCIFERASE
POLYMERASE CHAIN REACTION
RNA
VEINS