Upregulated circular RNA circ{sub 0}016760 indicates unfavorable prognosis in NSCLC and promotes cell progression through miR-1287/GAGE1 axis

Journal Article · Sat Sep 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.07.164· OSTI ID:23134310

Li, Yongsheng ^[1]; Hu, Jiahang ^[2]; Li, Lijuan ^[3]; Cai, Shengyan ^[2]; Zhang, Hengchun ^[4]; Zhu, Xianfeng ^[1]; Guan, Guijie; Dong, Xiangmei ^[3]

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1. Department of Imaging, Mudanjiang Medical University, Mudanjiang, 157000 (China)
2. Department of Imaging, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, 157000 (China)
3. Department of Pathology, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, 157000 (China)
4. Department of General Surgery, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, 157000 (China)

Non-small cell lung cancer (NSCLC) is an aggressive malignancy with increasing worldwide incidence and is characterized by dismal prognosis due to its early recurrence and metastasis. Accumulating evidence documented that aberrantly expressed circular RNAs (circRNAs) are critically involved in tumorigenesis. In the current study, we focused on a novel circRNA, circ{sub 0}016760 in NSCLC. qRT-PCR was carried out to determine the expression level of circ{sub 0}016760 in tissue samples and cell lines. The clinical value of circ{sub 0}016760 was also investigated. The functions of circ{sub 0}016760 was explored by CCK-8, colony formation, flow cytometry, Transwell and animal experiments. Luciferase reporter assays were conducted to investigate the association between circ{sub 0}016760 and miR-1287 and miR-1287 and GAGE1. We found that circ{sub 0}016760 was enhanced in NSCLC tissues and cells and the upregulation of circ{sub 0}016760 is associated with advanced TNM stages, lymph node metastasis and adverse prognosis in NSCLC patients. Moreover, circ{sub 0}016760 could significantly promote cell growth and metastatic properties and inhibit cell apoptosis in NSCLC cells. In mechanism, circ{sub 0}016760 functioned as a sponge for miR-1287 and regulated the expression of GAGE1. Subsequently, functional assays illustrated that the oncogenic properties of circ{sub 0}016760 is partly attribute to the regulation of miR-1287/GAGE1 axis. In summary, circ{sub 0}016760/miR-1287/GAGE1 signaling might play vital roles in the tumorigenesis and progression of NSCLC.

• OSTI ID: 23134310
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 503; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
COLONY FORMATION
LUCIFERASE
LUNGS
NEOPLASMS
PORIFERA
RNA