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Toxicokinetics and disposition of mercuric and cadmium chloride in channel catfish after intravascular administration

Conference ·
OSTI ID:230870
; ;  [1]
  1. Savannah River Ecology Lab., Aiken, SC (United States)
To better understand the distribution and elimination of inorganic mercury and cadmium in fish, {sup 203}HgCl{sub 2} and {sup 109}CdCl{sub 2} were administered to channel catfish (Ictalurus punctatus) via an intra-aortic cannula. Blood was serially removed from individual fish for at least 158 days after injection and the concentrations of {sup 203}Hg and {sup 109}Cd in whole blood, plasma and red blood cells (RBC) were determined. Additionally, the amount of {sup 203}Hg and {sup 109}Cd in the whole animal was serially determined by whole-body counting of the lightly anesthetized fish with a gamma spectroscopy system. Initially, the concentrations of {sup 203}Hg and {sup 109}Cd in blood and plasma declined rapidly and then slowly decreased with detectable amounts still present after 158 days. By 300 hrs post-injection, the majority of the {sup 203}Hg and {sup 109}Cd in the blood was associated with the RBC. The amount of {sup 203}Hg in the whole animal declined slowly with < 50% of the injected dose excreted after 158 days. {sup 109}Cd was eliminated very slowly with < 15% of the dose excreted after 230 days. The majority of {sup 203}Hg and {sup 109}Cd was associated with the liver, trunk and head kidneys. The {sup 203}Hg and {sup 109}Cd concentration time curves in plasma, RBC`s and the amount in the whole animal were fitted simultaneously to a 4 compartment toxicokinetic model to obtain estimates of the apparent volume of distribution and total body clearance.
OSTI ID:
230870
Report Number(s):
CONF-9511137--; ISBN 1-880611-03-1
Country of Publication:
United States
Language:
English

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