Adenovirus E1A TRRAP-targeting domain-mediated enhancement of MYC association with the NuA4 complex activates a panel of MYC target genes enriched for gene expression and ribosome biogenesis
Cellular transformation by adenovirus E1A requires targeting TRRAP, a scaffold protein which helps assemble histone acetyltransferase complexes, including the NuA4 complex. We recently reported that E1A and E1A 1–80 (N-terminal 80 aa) promote association of the proto-oncogene product MYC with the NuA4 complex. The E1A N-terminal TRRAP-targeting (ET) domain is required for E1A 1–80 to interact with the NuA4 complex. We demonstrate that an ET-MYC fusion associates with the NuA4 complex more efficiently than does MYC alone. Because MYC regulates genes for multiple cellular pathways, we performed global RNA-sequence analysis of cells expressing MYC or ET-MYC, and identified a panel of genes (262) preferentially activated by ET-MYC and significantly enriched in genes involved in gene expression and ribosome biogenesis, suggesting that E1A enhances MYC association with the NuA4 complex to activate a set of MYC target genes likely involved in cellular proliferation and cellular transformation by E1A and by MYC. - Highlights: • E1A N-terminal-MYC chimeric protein associates with the NuA4 complex efficiently. • A panel of MYC-target genes is preferentially activated by E1A N-terminal-MYC fusion. • This panel of genes may be activated by MYC association with NuA4 complex. • This panel of genes may be involved in E1A and MYC-mediated transformation.
- OSTI ID:
- 22722977
- Journal Information:
- Virology, Journal Name: Virology Vol. 512; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
E1A-dependent trans-activation of the human MYC promoter is mediated by the E2F factor
The adenovirus E4 gene, in addition to the E1A gene, is important for trans-activation of E2 transcription and for E2F activation
Interaction of CtBP with adenovirus E1A suppresses immortalization of primary epithelial cells and enhances virus replication during productive infection
Journal Article
·
Mon May 01 00:00:00 EDT 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5434023
The adenovirus E4 gene, in addition to the E1A gene, is important for trans-activation of E2 transcription and for E2F activation
Journal Article
·
Fri Sep 01 00:00:00 EDT 1989
· Journal of Virology; (USA)
·
OSTI ID:7194985
Interaction of CtBP with adenovirus E1A suppresses immortalization of primary epithelial cells and enhances virus replication during productive infection
Journal Article
·
Sun Sep 01 00:00:00 EDT 2013
· Virology
·
OSTI ID:22436638