Linking loss of sodium-iodide symporter expression to DNA damage

Journal Article · Sun May 15 04:00:00 EDT 2016 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2016.04.015· OSTI ID:22648579

Lyckesvärd, Madeleine Nordén ^[1]; Kapoor, Nirmal ^[2]; Ingeson-Carlsson, Camilla; Carlsson, Therese ^[1]; Karlsson, Jan-Olof ^[2]; Postgård, Per; Himmelman, Jakob; Forssell-Aronsson, Eva ^[3]; Hammarsten, Ola ^[4]; Nilsson, Mikael ^[1]

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1. Sahlgrenska Cancer Center, University of Gothenburg, Göteborg (Sweden)
2. Department of Medical Chemistry and Cell Biology, University of Gothenburg, Göteborg (Sweden)
3. Department of Radiation Physics, University of Gothenburg, Göteborg (Sweden)
4. Department of Clinical Chemistry, University of Gothenburg, Göteborg (Sweden)

Radiotherapy of thyroid cancer with I-131 is abrogated by inherent loss of radioiodine uptake due to loss of sodium iodide symporter (NIS) expression in poorly differentiated tumor cells. It is also known that ionizing radiation per se down-regulates NIS (the stunning effect), but the mechanism is unknown. Here we investigated whether loss of NIS-mediated iodide transport may be elicited by DNA damage. Calicheamicin, a fungal toxin that specifically cleaves double-stranded DNA, induced a full scale DNA damage response mediated by the ataxia-telangiectasia mutated (ATM) kinase in quiescent normal thyrocytes. At sublethal concentrations (<1 nM) calicheamicin blocked NIS mRNA expression and transepithelial iodide transport as stimulated by thyrotropin; loss of function occurred at a much faster rate than after I-131 irradiation. KU-55933, a selective ATM kinase inhibitor, partly rescued NIS expression and iodide transport in DNA-damaged cells. Prolonged ATM inhibition in healthy cells also repressed NIS-mediated iodide transport. ATM-dependent loss of iodide transport was counteracted by IGF-1. Together, these findings indicate that NIS, the major iodide transporter of the thyroid gland, is susceptible to DNA damage involving ATM-mediated mechanisms. This uncovers novel means of poor radioiodine uptake in thyroid cells subjected to extrinsic or intrinsic genotoxic stress. - Highlights: • DNA damage inhibits polarized iodide transport in normal thyroid cells. • Down-regulation of NIS expression is mediated by activation of the ATM kinase. • Long-term ATM inhibition also represses NIS-mediated iodide transport. • IGF-1 rescues NIS expression and iodide transport in DNA-damaged cells.

• OSTI ID: 22648579
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 1 Vol. 344; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ABUNDANCE
CONCENTRATION RATIO
DNA
DNA DAMAGES
ECOLOGICAL CONCENTRATION
INHIBITION
IODINE 131
IONIZING RADIATIONS
IRRADIATION
MESSENGER-RNA
NEOPLASMS
NICKEL SULFIDES
PHOSPHOTRANSFERASES
RADIOTHERAPY
REGULATIONS
SODIUM
SODIUM IODIDES
THYROID
THYROID CELLS
TOXINS
TUMOR CELLS
UPTAKE