GSE1 negative regulation by miR-489-5p promotes breast cancer cell proliferation and invasion

Journal Article · Fri Feb 26 04:00:00 EST 2016 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2016.01.168· OSTI ID:22594263

Chai, Peng ^[1]; Tian, Jingzhong ^[1]; Zhao, Deyin ^[2]; Zhang, Hongyan; Cui, Jian ^[1]; Ding, Keshuo ^[3]; Liu, Bin ^[4]

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1. Department of General Surgery, The People's Hospital of Bozhou, Bozhou 236800 (China)
2. Dept of General Surgery, The Hospital of Suzhou, Suzhou 234000 (China)
3. Department of Pathology, Anhui Medical University, Hefei 230022 (China)
4. Dept of Vascular Surgery, The First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China)

Gse1 coiled-coil protein (GSE1), also known as KIAA0182, is a proline rich protein. However, the function of GSE1 is largely unknown. In this study, we reported that GSE1 is overexpression in breast cancer and silencing of GSE1 significantly suppressed breast cancer cells proliferation, migration and invasion. Furthermore, GSE1 was identified as a direct target of miR-489-5p, which is significantly reduced in breast cancer tissues. In addition, forced expression of miR-489-5p suppressed breast cancer cells proliferation, migration and invasion. Moreover, depletion of GSE1 by siRNAs significantly abrogated the enhanced proliferation, migration and invasion of breast cancer cells consequent to miR-489-5p depletion. Taken together, these findings suggest that GSE1 may function as a novel oncogene in breast cancer and it can be regulated by miR-489-5p. - Highlights: • GSE1 is overexpressed in breast cancer and increased GSE1 expression predicts poor prognosis in breast cancer patients. • Knockdown of GSE1 inhibits breast cancer cell proliferation, migration and invasion. • GSE1 is a direct target of miR-489-5p. • Forced expression of miR-489-5p inhibits breast cancer cell proliferation, migration and invasion.

• OSTI ID: 22594263
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 471; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL TISSUES
CELL PROLIFERATION
MAMMARY GLANDS
NEOPLASMS
ONCOGENES
PATIENTS
PROLINE
PROTEINS