MiR-30a-5p inhibits osteosarcoma cell proliferation and migration by targeting FOXD1

Journal Article · Sat Sep 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.06.121· OSTI ID:23134357

Tao, Jun ^[1]; Cong, Haibo; Wang, Hongyan; Zhang, Daoqiang; Liu, Chuanjie; Chu, Hongxia; Qing, Qianfeng ^[2]; Wang, Kunzheng ^[1]

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1. Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710004 (China)
2. Weihai City Key Laboratory of Autoimmunity of Weihai Central Hospital, Weihai, Shandong Province, 264400 (China)

Highlights: • FOXD1 is overexpressed in osteosarcoma while MiR-30a-5p is lowexpressed. • MiR-30a-5p directly regulates FOXD1 by binding its 3′-UTR. • Inhibition of FOXD1 suppresses osteosarcoma cell migration and invasion. • Inhibition of FOXD1 suppresses osteosarcoma cell proliferation in vitro and in vivo. Despite a number of studies have emphasized the extensive role of microRNA (miRNA) in the development of multiple cancers, the role of miR-30a-5p in the progression of osteosarcoma (OS) and the underlying mechanism are still limited. We detected the expression level of MiR-30a-5p and forkhead box D1 (FOXD1) in Clinical OS specimens and found that miR-30a-5p was significantly decreased while FOXD1 was markedly increased. Dual luciferase assay confirmed that FOXD1 was directly regulated by miR-30a-5p. In vitro assay showed that inhibitior of FOXD1 suppressed cell proliferation, migration and invasion in MG63 and U2OS cells, while overexpression of FOXD1 promoted OS cell proliferation and migration. In vivo assay further showed the inhibition of tumor growth after knockdown of FOXD1. These results suggested that FOXD1 might play key roles in OS development and progression, and was negatively regulated by miR-30a-5p in OS.

• OSTI ID: 23134357
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 503; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
LUCIFERASE
OSTEOSARCOMAS