miR-125b-5p inhibits breast cancer cell proliferation, migration and invasion by targeting KIAA1522

Journal Article · Sat Sep 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.08.172· OSTI ID:23103532

Li, Yongzhen; Wang, Yongxia ^[1]; Fan, Hongzhe ^[2]; Zhang, Zheying; Li, Na ^[1]

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1. Department of Pathology, Xinxiang Medical University, No. 601 Jinsui Avenue, Hongqi District, Xinxiang, 453003 (China)
2. Cardiothoracic Surgery, The Third Affiliated Hospital of Xinxiang Medical University, East Hualan Avenue, Hongqi District, Xinxiang, 453000 (China)

Highlights: • miR-125b-5p expression was found downregulated in breast cancer. • We explored the tumor suppressive role of miR-125b-5p in breast cancer. • KIAA1522 was reported to function as oncogene in breast cancer. • KIAA1522 was a direct target of miR-125b-5p in breast cancer. Abnormal gene expression due to the dysregulation of microRNAs (miRNAs) often occurred in the initiation or progression of cancers. The aim of this present study was to investigate the function role of miR-125b-5p in breast cancer (BC). Expression levels of miR-125b-5p were determined by quantitative Real-time PCR. Biological functions of miR-125b-5p in the progression of BC were investigated with a series of in vitro experiments including cell counting kit-8 assay, colony formation assay, wound-healing assay and transwell invasion assay. The target of miR-125b-5p in BC was validated by luciferase activity reporter assay and western blot assay. We found miR-125b-5p expression was significantly reduced in BC cell lines compared to the normal breast epithelial cell line. Functional assays showed that cell proliferation, colony formation ability, cell migration, and cell invasion can be suppressed by miR-125b-5p overexpression. Besides, KIAA1522 was validated as a direct target of miR-125b-5p in BC. Collectively, our study showed that miR-125b-5p functions as a tumor suppressor and regulates BC progression through targeting KIAA1522.

• OSTI ID: 23103532
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 504; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
COLONY FORMATION
LUCIFERASE
MAMMARY GLANDS
NEOPLASMS
ONCOGENES
WOUNDS