Epidermis–dermis junction as a novel location for bone marrow-derived cells to reside in response to ionizing radiation

Journal Article · Fri Jun 12 04:00:00 EDT 2015 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2015.04.094· OSTI ID:22462084

Okano, Junko ^[1]; Kojima, Hideto; Katagi, Miwako ^[2]; Nakae, Yuki ^[3]; Terashima, Tomoya ^[2]; Nakagawa, Takahiko ^[4]; Kurakane, Takeshi; Okamoto, Naoki; Morohashi, Keita ^[1]; Maegawa, Hiroshi ^[3]; Udagawa, Jun ^[1]

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1. Division of Anatomy and Cell Biology, Shiga University of Medical Science, Shiga (Japan)
2. Department of Stem Cell Biology and Regenerative Medicine, Shiga University of Medical Science, Shiga (Japan)
3. Department of Internal Medicine, Shiga University of Medical Science, Shiga (Japan)
4. TMK Project, Medical Innovation Center, Kyoto University Graduate School of Medicine, Kyoto (Japan)

Bone marrow-derived cells (BMDCs) can migrate into the various organs in the mice irradiated by ionizing radiation (IR). However, it may not be the case in the skin. While IR is used for bone marrow (BM) transplantation, studying with the epidermal sheets demonstrated that the BMDC recruitment is extraordinarily rare in epidermis in the mouse. Herein, using the chimera mice with BM from green fluorescent protein (GFP) transgenic mice, we simply examined if BMDCs migrate into any layers in the total skin, as opposed to the epidermal sheets, in response to IR. Interestingly, we identified the presence of GFP-positive (GFP{sup +}) cells in the epidermis-dermis junction in the total skin sections although the epidermal cell sheets failed to have any GFP cells. To examine a possibility that the cells in the junction could be mechanically dissociated during separating epidermal sheets, we then salvaged such dissociated cells and examined its characteristics. Surprisingly, some GFP{sup +} cells were found in the salvaged cells, indicating that these cells could be derived from BM. In addition, such BMDCs were also associated with inflammation in the junction. In conclusion, BMDCs can migrate to and reside in the epidermis-dermis junction after IR. - Highlights: • Bone marrow-derived cells (BMDCs) migrate in the epidermis due to ionizing radiation (IR). • BMDCs dissociate from the epidermis-dermis junction in preparing epidermal sheets. • The doses of IR determine the location and the number of migrating BMDCs in the skin.

• OSTI ID: 22462084
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 461; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BONE MARROW
CHIMERAS
DOSES
EPIDERMIS
FLUORESCENCE
INFLAMMATION
IONIZING RADIATIONS
IRRADIATION
LAYERS
PROTEINS
SHEETS
TRANSGENIC MICE