Thy-1+ dendritic cells in murine epidermis are bone marrow-derived
Thy-1+, Ly-5+ dendritic cells have recently been described as a resident cell population in murine epidermis, but their ontogeny and function are unknown. The origin and turnover of epidermal Thy-1+ cells utilizing chimeric mice were investigated. Lethally x-irradiated AKR/J (Thy-1.1+) and AKR/Cum (Thy-1.2+) mice were reconstituted with allogeneic bone marrow cells with or without thymocytes from congenic AKR/Cum or AKR/J mice, respectively. The density of residual indigenous Thy-1.1+ cells in AKR/J chimeras and Thy-1.2+ cells in AKR/Cum chimeras was substantially reduced following x-irradiation, as determined by immunofluorescence staining of epidermal sheets. Epidermal repopulation by allogeneic Thy-1+ dendritic epidermal cells was first observed at 5 weeks in AKR/J chimeras and at 7 weeks in AKR/Cum chimeras and progressed slowly. Repopulation was not enhanced by increasing the number of allogeneic bone marrow cells injected from 2 X 10(7) to 10(8) cells or by the addition of 8 X 10(7) allogeneic thymocytes to the donor inoculate. Epidermal repopulation by allogeneic Thy-1.2+ cells was not seen in AKR/J mice reconstituted with syngeneic bone marrow cells and allogeneic Thy-1.2+ AKR/Cum thymocytes. Taken together, these results indicate that Thy-1+ dendritic epidermal cells are derived from the bone marrow and suggest that they are not related to conventional peripheral T-lymphocytes.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 6347739
- Journal Information:
- J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 83:1; ISSN JIDEA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BIOLOGY
BODY
BONE MARROW
CELL CULTURES
CELL PROLIFERATION
CHIMERAS
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
EPIDERMIS
EPITHELIUM
FLUORESCENCE SPECTROSCOPY
HEMATOPOIETIC SYSTEM
IONIZING RADIATIONS
IRRADIATION
LETHAL IRRADIATION
MAMMALS
MICE
MOSAICISM
ONTOGENESIS
ORGANS
RADIATION CHIMERAS
RADIATION EFFECTS
RADIATIONS
RADIOBIOLOGY
RECOVERY
RODENTS
SKIN
SOMATIC CELLS
SPECTROSCOPY
THYMOCYTES
TISSUES
VERTEBRATES
X RADIATION