Direct electrochemistry and electrocatalysis of myoglobin immobilized on zirconia/multi-walled carbon nanotube nanocomposite

Journal Article · Wed Dec 15 04:00:00 EST 2010 · Materials Research Bulletin

DOI:https://doi.org/10.1016/J.MATERRESBULL.2010.09.016· OSTI ID:22209949

Liang, Ruping; Deng, Minqiang; Cui, Sanguan; Chen, Hong ^[1]; Qiu, Jianding ^[1]

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1. Department of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang 330031 (China)

Zirconia/multi-walled carbon nanotube (ZrO{sub 2}/MWCNT) nanocomposite was prepared by hydrothermal treatment of MWCNTs in ZrOCl{sub 2}.8H{sub 2}O aqueous solution. The morphology and structure of the synthesized ZrO{sub 2}/MWCNT nanocomposite were characterized by transmission electron microscopy and X-ray diffraction analysis. It was found that ZrO{sub 2} nanoparticles homogeneously distributed on the sidewall of MWCNTs. Myoglobin (Mb), as a model protein to investigate the nanocomposite, was immobilized on ZrO{sub 2}/MWCNT nanocomposite. Ultraviolet-visible spectroscopy and electrochemical measurements showed that the nanocomposite could retain the bioactivity of the immobilized Mb to a large extent. The Mb immobilized in the composite showed excellent direct electrochemistry and electrocatalytic activity to the reduction of hydrogen peroxide (H{sub 2}O{sub 2}). The linear response range of the biosensor to H{sub 2}O{sub 2} concentration was from 1.0 to 116.0 {mu}M with the limit of detection of 0.53 {mu}M (S/N = 3). The ZrO{sub 2}/MWCNT nanocomposite provided a good biocompatible matrix for protein immobilization and biosensors preparation.

• OSTI ID: 22209949
• Journal Information: Materials Research Bulletin, Journal Name: Materials Research Bulletin Journal Issue: 12 Vol. 45; ISSN MRBUAC; ISSN 0025-5408
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
AQUEOUS SOLUTIONS
CARBON NANOTUBES
HYDROGEN PEROXIDE
MYOGLOBIN
SPECTROSCOPY
SYNTHESIS
TRANSMISSION ELECTRON MICROSCOPY
X-RAY DIFFRACTION
ZIRCONIUM OXIDES