Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing

Journal Article · Sun Nov 01 04:00:00 EST 2009 · Biosensors and Bioelectronics, 25(4):901-905

DOI:https://doi.org/10.1016/j.bios.2009.09.004· OSTI ID:969608

Kang, Xinhuang; Wang, Jun; Wu, Hong; Aksay, Ilhan A; Liu, Jun; Lin, Yuehe

Direct electrochemistry of a glucose oxidase (GOD)/graphene/chitosan nanocomposite was studied. The immobilized enzyme retains its bioactivity, exhibits a surface confined, reversible two-proton and two-electron transfer reaction, and has good stability, activity and a fast heterogeneous electron transfer rate with the rate constant (ks) of 2.83 s-1. A much higher enzyme loading (1.12 × 10-9 mol/cm2) is obtained as compared to the bare glass carbon surface. This GOD/graphene/chitosan nanocomposite film can be used for sensitive detection of glucose. The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 μA mM-1 cm-2) as compared with other nanostructured supports. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of graphene, and good biocompatibility of chitosan, which enhances the enzyme absorption and promotes direct electron transfer between redox enzymes and the surface of electrodes.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 969608
• Report Number(s): PNNL-SA-68233; 24822
• Journal Information: Biosensors and Bioelectronics, 25(4):901-905, Journal Name: Biosensors and Bioelectronics, 25(4):901-905 Journal Issue: 4 Vol. 25
• Country of Publication: United States
• Language: English

Related Subjects

73 NUCLEAR PHYSICS AND RADIATION PHYSICS
ABSORPTION
CARBON
Chitosan
DETECTION
Direct electron transfer
ELECTROCHEMISTRY
ELECTRODES
ELECTRON TRANSFER
ENZYMES
Environmental Molecular Sciences Laboratory
GLASS
GLUCOSE
Glucose oxidase
Graphene
IMMOBILIZED ENZYMES
OXIDASES
PERFORMANCE
SENSITIVITY
STABILITY
Sensor
TRANSFER REACTIONS