Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin

Journal Article · Thu Oct 25 04:00:00 EDT 2012 · Virology

DOI:https://doi.org/10.1016/J.VIROL.2012.07.007· OSTI ID:22150075

Vazquez-Iglesias, Lorena; Lostale-Seijo, Irene; Martinez-Costas, Jose ^[1]; Benavente, Javier ^[1]

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1. Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, y Centro Singular de Investigacion en Quimica Biologica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, 15782-Santiago de Compostela (Spain)

A comparative analysis of the intracellular distribution of avian reovirus (ARV) core protein sigmaA in cells of avian and mammalian origin revealed that, whereas the viral protein accumulates in the cytoplasm and nucleolus of avian cells, most sigmaA concentrates in the nucleoplasm of mammalian cells in tight association with the insoluble nuclear matrix fraction. Our results further showed that sigmaA becomes arrested in the nucleoplasm of mammalian cells via association with mammalian cell-specific factors and that this association prevents nucleolar targeting. Inhibition of RNA polymerase II activity, but not of RNA polymerase I activity, in infected mammalian cells induces nucleus-to-cytoplasm sigmaA translocation through a CRM1- and RanGTP-dependent mechanism, yet a heterokaryon assay suggests that sigmaA does not shuttle between the nucleus and cytoplasm. The scarcity of sigmaA in cytoplasmic viral factories of infected mammalian cells could be one of the factors contributing to limited ARV replication in mammalian cells.

• OSTI ID: 22150075
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 432; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CYTOPLASM
INHIBITION
NUCLEAR MATRIX
PROTEINS
RNA
TRANSLOCATION