Identification of a nuclear export signal in the KSHV latent protein LANA2 mediating its export from the nucleus

Journal Article · Tue Nov 15 04:00:00 EST 2005 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2005.08.022· OSTI ID:20717687

Munoz-Fontela, C ^[1]; Collado, M ^[2]; Rodriguez, E ^[1]; Garcia, M A ^[3]; Alvarez-Barrientos, A ^[4]; Arroyo, J ^[1]; Nombela, C ^[1]; Rivas, C ^[1]

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1. Departamento de Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramon y Cajal sn, 28040 Madrid (Spain)
2. Molecular Oncology Program, Centro Nacional de Investigaciones Oncologicas, Melchor Fernandez Almagro 3, 28029 Madrid (Spain)
3. Centro Nacional de Biotecnologia (CNB), Universidad Autonoma de Madrid, 28049 Madrid (Spain)
4. Unidad de Citometria, Fundacion Centro Nacional de Investigaciones Cardiovasculares, Instituto de Salud Carlos III, Ronda de Poniente 5, Tres Cantos, 28760 Madrid (Spain)

LANA2 is a latent protein detected in Kaposi's sarcoma-associated herpesvirus (KSHV)-infected B cells that inhibits p53-dependent transcriptional transactivation and apoptosis and PKR-dependent apoptosis, suggesting an important role in the transforming activity of the virus. It has been reported that LANA2 localizes into the nucleus of both KSHV-infected B cells and transiently transfected HeLa cells. In this study, we show that LANA2 is a nucleocytoplasmic shuttling protein that requires a Rev-type nuclear export signal located in the C-terminus to direct the protein to the cytoplasm, through an association with the export receptor CRM1. In addition, a functional protein kinase B (PKB)/Akt phosphorylation motif partially overlapping with the nuclear export signal was identified. Nuclear exclusion of LANA2 was negatively regulated by the phosphorylation of threonine 564 by Akt. The ability of LANA2 to shuttle between nucleus and cytoplasm has implications for the function of this viral protein.

• OSTI ID: 20717687
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 1 Vol. 311; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
CELL NUCLEI
CYTOPLASM
HELA CELLS
PHOSPHORYLATION
RABBIT TUBES
RECEPTORS
SARCOMAS
THREONINE
VIRUSES