Characterization of molecular determinants for nucleocytoplasmic shuttling of PRV UL54

Journal Article · Thu Sep 01 04:00:00 EDT 2011 · Virology

OSTI ID:21587887

Meili, Li ^[1]; Shuai, Wang ^[2]; Mingsheng, Cai; Hong, Guo ^[1]

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1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071 (China)
2. Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071 (China)

The pseudorabies virus (PRV) early protein UL54 is a homologue of the herpes simplex virus 1 (HSV-1) immediate-early protein ICP27, which is a multifunctional protein and essential for HSV-1 infection. To determine if UL54 might shuttle between the nucleus and cytoplasm, as has been shown for its homologues in human herpesviruses, the molecular determinants for its nucleocytoplasmic shuttling were investigated. Heterokaryon assays demonstrated that UL54 was a nucleocytoplasmic shuttling protein and this property could not be blocked by leptomycin B, an inhibitor of chromosome region maintenance 1 (CRM1). However, TAP/NXF1 promoted the nuclear export of UL54 and interacted with UL54, suggesting that UL54 shuttles between the nucleus and the cytoplasm via a TAP/NXF1, but not CRM1, dependent nuclear export pathway. Furthermore, UL54 was demonstrated to target to the nucleus through a classic Ran-, importin {beta}1- and {alpha}5-dependent nuclear import mechanism.

• OSTI ID: 21587887
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 417; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CONSTITUENTS
CHROMOSOMES
CYTOPLASM
DISEASES
HERPES SIMPLEX
INFECTIOUS DISEASES
MICROORGANISMS
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RABBIT TUBES
REACTION PRODUCT TRANSPORT SYSTEMS
REACTOR COMPONENTS
REACTOR EXPERIMENTAL FACILITIES
SKIN DISEASES
VIRAL DISEASES
VIRUSES