SR proteins SRp20 and 9G8 contribute to efficient export of herpes simplex virus 1 mRNAs

Journal Article · Sat Jun 05 04:00:00 EDT 2010 · Virology

DOI:https://doi.org/10.1016/j.virol.2010.02.023· OSTI ID:21357618

Escudero-Paunetto, Laurimar; Ling, Li; Hernandez, Felicia P ^[1]

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1. Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92697 (United States)

Herpes simplex virus 1 (HSV-1) mRNAs are exported to the cytoplasm through the export receptor TAP/NFX1. HSV-1 multifunctional protein ICP27 interacts with TAP/NXF1, binds viral RNAs, and is required for efficient viral RNA export. In ICP27 mutant infections, viral RNA export is reduced but not ablated, indicating that other export adaptors can aid in viral RNA export. Export adaptor protein Aly/REF is recruited to viral replication compartments, however, Aly/REF knockdown has little effect on viral RNA export. SR proteins SRp20 and 9G8 interact with TAP/NXF1 and mediate export of some cellular RNAs. We report that siRNA knockdown of SRp20 or 9G8 resulted in about a 10 fold decrease in virus yields and in nuclear accumulation of poly(A+) RNA. In infected cells depleted of SRp20, newly transcribed Bromouridine-labeled RNA also accumulated in the nucleus. We conclude that SRp20 and 9G8 contribute to HSV-1 RNA export.

• OSTI ID: 21357618
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 401; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BUILDUP
CELL CONSTITUENTS
CYTOPLASM
DISEASES
HERPES SIMPLEX
INFECTIOUS DISEASES
MEMBRANE PROTEINS
MESSENGER-RNA
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RECEPTORS
RNA
SKIN DISEASES
VIRAL DISEASES
VIRUSES