Nucleocytoplasmic shuttling of the HSV-2 serine/threonine kinase Us3

Journal Article · Mon Aug 15 04:00:00 EDT 2011 · Virology

DOI:https://doi.org/10.1016/j.virol.2011.06.011· OSTI ID:21587875

Finnen, Renee L; Johnston, Susan M; Neron, Casey E

The alphaherpesvirus serine/threonine kinase Us3 plays diverse roles in virus multiplication and modifies both nuclear and cytoplasmic substrates. We recently reported that treatment of HSV-2 Us3-transfected and HSV-2-infected cells with leptomycin B, an inhibitor of nuclear export mediated by interaction of chromosomal regional maintenance protein (CRM1) with leucine rich nuclear export signals (NESs), resulted in nuclear trapping of Us3. Here, we utilized fluorescence loss in photobleaching to monitor nuclear export of HSV-2 Us3 and confirm that this process proceeds solely via a CRM1-mediated mechanism. Analysis of deletion derivatives of HSV-2 Us3 fused to a nuclear export reporter protein implicated the involvement of NES-like sequences in nuclear export. However, nuclear trapping of HSV-2 Us3 proteins carrying mutations in these potential NESs was not observed, indicating that these sequences are not functional in the context of full-length protein. Our analyses also revealed previously unidentified regions of HSV-2 Us3 that contribute to its kinase activity.

• OSTI ID: 21587875
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 417; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
EMISSION
FLUORESCENCE
HYDROXY ACIDS
INTERACTIONS
LEUCINE
LUMINESCENCE
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOTON EMISSION
PROTEINS
SERINE
SUBSTRATES
THREONINE