The BRO proteins of Bombyx mori nucleopolyhedrovirus are nucleocytoplasmic shuttling proteins that utilize the CRM1-mediated nuclear export pathway

Journal Article · Tue Jun 20 04:00:00 EDT 2006 · Virology

DOI:https://doi.org/10.1016/j.virol.2006.01.008· OSTI ID:20850522

Kang, Won Kyung ^[1]; Kurihara, Masaaki ^[1]; Matsumoto, Shogo ^[1]

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1. Molecular Entomology Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198 (Japan)

The BRO proteins of Bombyx mori nucleopolyhedrovirus (BmNPV) display a biphasic pattern of intracellular localization during infection. At early times, they reside in the nucleus but then show both cytoplasmic and nuclear localization as the infection proceeds. Therefore, we examined the possibility of nuclear export. Using inhibitors, we reveal that BmNPV BRO proteins shuttle between the nucleus and cytoplasm. Mutations on the leucine-rich region of BRO proteins resulted in nuclear accumulation of transiently expressed proteins, suggesting that this region functions as a CRM1-dependent nuclear export signal (NES). On the contrary, mutant BRO-D with an altered NES did not show nuclear accumulation in infected cells, although protein production seemed to be reduced. RT-PCR analysis showed that the lower level of protein production was due to a reduction in RNA synthesis. Taken together, our results suggest that BRO proteins are nucleocytoplasmic shuttling proteins that utilize the CRM1-mediated nuclear export pathway.

• OSTI ID: 20850522
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 350; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOSYNTHESIS
CYTOPLASM
LEUCINE
MUTANTS
MUTATIONS
POLYMERASE CHAIN REACTION
PROTEINS
RABBIT TUBES
RNA
SILKWORM