The use of specific binding of peptide-nucleic acid to DNA in the {open_quotes}Achilles heel{close_quotes} method
- Institute of Molecular Genetics, Moscow (Russian Federation)
The `Achilles heel` method (AHM) is used in the design of random-cleavage restriction endonucleases. These promising compounds can be widely used in practice, particularly for genomic DNA mapping. DNA is complexed with a site-specific DNA-binding reagent (protein or oligonucleotide) and then treated with methylase. The methylation sites overlapping with or adjacent to the DNA-binding sites of the reagent are protected from methylation. Thereafter, methylase is inactivated, the DNA-binding reagent is removed, and DNA is cleaved by the restriction endonuclease corresponding to the methylase used. As a result, DNA is cleaved only at those restriction sites that were protected by the DNA-binding reagent from methylation. Until now, proteins or oligonucleotides were used as site-specific DNA-binding reagents. Here, we report on the use of a peptide-nucleic acid (PNA) in AHM. 7 refs., 4 figs.
- OSTI ID:
- 209970
- Journal Information:
- Doklady Biochemistry, Journal Name: Doklady Biochemistry Vol. 344; ISSN DBIOAM; ISSN 0012-4958
- Country of Publication:
- United States
- Language:
- English
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