Site specific cleavage of DNA
A method is described for site specific cleavage of a double-stranded DNA molecule at a recognition sequence not less than eight base pairs long, comprising: (a) contacting the double-stranded DNA molecule, which has at least two methylase recognition sequences, with at least one sequence specific methylase to produce a methylated double-stranded DNA molecule having a newly created DNA specific methylation dependent restriction endonuclease recognition sequence which partly overlaps with at least two of the methylase recognition sequences and the overlapping sequences having a combined length of not less than eight base pairs; and (b) contacting the methylated double-stranded DNA molecule with a methylation dependent DNA specific restriction endonuclease, under conditions sufficient for cleavage of the double-stranded DNA molecules by the restriction endonuclease.
- Assignee:
- Univ. of Georgia Research Center, Athens, GA
- Patent Number(s):
- US 4808525
- OSTI ID:
- 5826906
- Country of Publication:
- United States
- Language:
- English
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