Involvement of UL24 in herpes-simplex-virus-1-induced dispersal of nucleolin

Journal Article · Thu Jul 05 04:00:00 EDT 2007 · Virology

DOI:https://doi.org/10.1016/j.virol.2007.01.028· OSTI ID:20977039

Lymberopoulos, Maria H ^[1]; Pearson, Angela ^[1]

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1. INRS-Institut Armand-Frappier, Universite du Quebec, 531 boul. des Prairies, Laval, Quebec, H7V 1B7 (Canada)

UL24 of herpes simplex virus 1 is important for efficient viral replication, but its function is unknown. We generated a recombinant virus, vHA-UL24, encoding UL24 with an N-terminal hemagglutinin tag. By indirect immunofluorescence at 9 h post-infection (hpi), we detected HA-UL24 in nuclear foci and in cytoplasmic speckles. HA-UL24 partially co-localized with nucleolin, but not with ICP8 or coilin, markers for nucleoli, viral replication compartments, and Cajal bodies respectively. HA-UL24 staining was often juxtaposed to that of another nucleolar protein, fibrillarin. Analysis of HSV-1-induced nucleolar modifications revealed that by 18 hpi, nucleolin staining had dispersed, and fibrillarin staining went from clusters of small spots to a few separate but prominent spots. Fibrillarin redistribution appeared to be independent of UL24. In contrast, cells infected with a UL24-deficient virus retained foci of nucleolin staining. Our results demonstrate involvement of UL24 in dispersal of nucleolin during infection.

• OSTI ID: 20977039
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 363; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
HERPES SIMPLEX
MODIFICATIONS
NUCLEOLI
PROTEINS
VIRUSES