High molecular weight adiponectin reduces apolipoprotein B and E release in human hepatocytes

Journal Article · Fri Jan 12 04:00:00 EST 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.11.058· OSTI ID:20857960

Neumeier, Markus ^[1]; Sigruener, Alexander ^[2]; Eggenhofer, Elke ^[1]; Weigert, Johanna ^[1]; Weiss, Thomas S ^[3]; Schaeffler, Andreas ^[1]; Schlitt, Hans J ^[4]; Aslanidis, Charalampos ^[2]; Piso, Pompiliu ^[4]; Langmann, Thomas ^[2]; Schmitz, Gerd ^[2]; Schoelmerich, Juergen ^[1]; Buechler, Christa ^[1]

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1. Department of Internal Medicine I, Regensburg University Medical Center, D-93042 Regensburg (Germany)
2. Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, D-93042 Regensburg (Germany)
3. Center for Liver Cell Research, University of Regensburg, D-93042 Regensburg (Germany)
4. Department of Surgery, University of Regensburg, D-93042 Regensburg (Germany)

Low circulating levels of high molecular weight adiponectin (HMW-Apm) have been linked to dyslipidaemia and systemic HMW-Apm negatively correlates with very low density lipoprotein (VLDL), apolipoprotein B (ApoB), and ApoE and is positively associated with ApoA-I. Therefore, it was investigated whether HMW-Apm alters the hepatic synthesis of ApoB, ApoE, and ApoA-I or the activity of the hepatic ATP-binding cassette transporter A1 (ABCA1), as the main determinant of plasma HDL. HMW-Apm reduces hepatic ApoB and ApoE release whereas ABCA1 protein, activity and ApoA-I were not altered. Global gene expression analysis revealed that hepatic nuclear factor 4-{alpha} (HNF4-{alpha}) and HNF4-{alpha} regulated genes like ApoB are downregulated by HMW-Apm and this was confirmed at the mRNA and protein level. Therefore it is concluded that HMW-adiponectin may ameliorate dyslipidaemia by reducing the hepatic release of ApoB and ApoE, whereas ABCA1 function and ApoA-I secretion are not influenced.

• OSTI ID: 20857960
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 352; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ATP
BIOSYNTHESIS
GENES
LIPOPROTEINS
LIVER
LIVER CELLS
MOLECULAR WEIGHT
SECRETION