Synthesis of HDL apolipoproteins by rat hepatocytes

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7171090

Hussain, M M; Kelley, M; Zannis, V I

The authors have used 2D-PAGE to study the synthesis, intracellular modification, and secretion of rat HDL apolipoproteins by primary cultures of rat hepatocytes. ApoA-IV, apoA-II and apoE synthesized after a 10 min pulse with /sup 35/S-methionine coincided on 2D-gels with their corresponding plasma forms and they were not modified further intracellularly or following secretion. A fraction (< 10%) of apoE was modified intracellularly to minor isoprotein forms that were insensitive to neuraminidase treatment. These later forms also constituted a minor component of the secreted and plasma rat apoE. The intracellular and newly secreted apoA-I differed from its plasma counterpart by -1 charge as described previously. The intracellular forms of rat apoA-I, apoA-IV and unmodified apoE differed from the products of cell free translation of rat liver mRNA by +1 charge. Their findings (a) establish the charge relationship between nascent and plasma rat apolipoproteins, (b) indicate that rat apoA-I, apoA-II and apoA-IV are not modified intracellularly, (c) suggest that there is a difference in the post-translational modification patterns between the rat and human hepatic apoE.

• Research Organization: Boston Univ. Medical Center, MA
• OSTI ID: 7171090
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIPOTROPIC FACTORS
LIVER CELLS
MAMMALS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SECRETION
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES