Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts

Journal Article · Fri Oct 27 04:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.08.137· OSTI ID:20854541

Ryoo, Zae Young ^[1]; Jung, Boo Kyoung ^[1]; Lee, Sang Ryeul ^[1]; Kim, Myoung Ok ^[1]; Kim, Sung Hyun ^[1]; Kim, Hyo Jin ^[2]; Ahn, Jung Yong ^[2]; Lee, Tae-Hoon ^[3]; Cho, Youl Hee ^[4]; Park, Jae Hak ^[5]; Kim, Jin Kyeoung ^[2]

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1. School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of)
2. CHA Research Institute, Pochon CHA University, Sungnam 463-836 (Korea, Republic of)
3. Department of Oral Biochemistry, College of Dentistry, Chonnam National University, Gwangju 500-757 (Korea, Republic of)
4. Department of Medical Genetics, College of Medicine, Hanyang University, Seoul 133-791 (Korea, Republic of)
5. Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 110-799 (Korea, Republic of)

Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.

• OSTI ID: 20854541
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 349; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AGAR
CELL CYCLE
CELL PROLIFERATION
FIBROBLASTS
GENES
IN VIVO
MICE
NEOPLASMS
PHENOTYPE
PROTEINS