Assessment of the Biological Impact of Engineered Nanomaterials Using Mass Spectrometry-based MultiOmics Approaches

Book · Mon May 15 04:00:00 EDT 2023

DOI:https://doi.org/10.1002/9781119896258.ch15· OSTI ID:1985980

Day, Nicholas J. ^[1]; Zhang, Tong ^[1]; Gaffrey, Matthew J. ^[1]; Thrall, Brian D. ^[1]; Qian, Weijun ^[1]

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1. BATTELLE (PACIFIC NW LAB)

The widespread use of engineered nanomaterials (ENMs) has expanded further than our understanding of their toxicity, prompting research into the biological responses against exposure to such materials. Genomics and transcriptomics have been extensively used to evaluate the biological effects of exposure to various ENMs. By determining gene activities, these studies provided valuable information to infer how cells respond to the toxicological effects of many ENMs. The application of mass spectrometry (MS)-based omics tools, such as proteomics, lipidomics, and metabolomics, offer post-genomic perspectives of what cellular processes are altered. Individually, these technologies have revealed the proteome, lipidome, and metabolome landscape upon exposure to ENMs. Together, these approaches demonstrate the ENM-induced adaptation in a broad range of cellular processes at multiple levels and the complexity of ENM-cell interactions. As a result, integrating multiple layers of MS-based omics data is trending to complement genomics data. In this review chapter, we discuss the applications of these tools for a comprehensive systems-level characterization of the biological responses induced by engineered nanomaterials.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1985980
• Report Number(s): PNNL-SA-177428
• Country of Publication: United States
• Language: English

Related Subjects

Engineered nanomaterials
Proteomics
Metabolomics
Lipidomics
Multi-Omics