Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials

Zhang, Tong; Gaffrey, Matthew J.; Thrall, Brian D.; Qian, Wei-Jun

doi:10.1007/S00216-018-1168-6

Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials

Journal Article · Fri Jun 08 00:00:00 EDT 2018 · Analytical and Bioanalytical Chemistry

DOI:https://doi.org/10.1007/S00216-018-1168-6· OSTI ID:1457755

Zhang, Tong ^[1]; Gaffrey, Matthew J. ^[1]; Thrall, Brian D. ^[1]; ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division

We present that the widespread use of engineered nanomaterials or nanotechnology makes the characterization of biological responses to nanomaterials an important area of research. The application of omics approaches, such as mass spectrometry-based proteomics, has revealed new insights into the cellular responses of exposure to nanomaterials, including how nanomaterials interact and alter cellular pathways. In addition, exposure to engineered nanomaterials often leads to the generation of reactive oxygen species and cellular oxidative stress, which implicates a redox-dependent regulation of cellular responses under such conditions. Here, in this review, we discuss quantitative proteomics-based approaches, with an emphasis on redox proteomics, as a tool for system-level characterization of the biological responses induced by engineered nanomaterials.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1457755

Alternate ID(s):: OSTI ID: 1503587

Report Number(s):: PNNL-SA--133275; PII: 1168

Journal Information:: Analytical and Bioanalytical Chemistry, Journal Name: Analytical and Bioanalytical Chemistry Journal Issue: 24 Vol. 410; ISSN 1618-2642

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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