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Megahertz serial crystallography

Journal Article · · Nature Communications
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Here, the new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE; USDOE National Nuclear Security Administration (NNSA)
Contributing Organization:
DESY
Grant/Contract Number:
AC02-76SF00515; AC52-07NA27344
OSTI ID:
1490639
Alternate ID(s):
OSTI ID: 1959486
Report Number(s):
LLNL-JRNL-843452; PII: 6156
Journal Information:
Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 9; ISSN 2041-1723
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (54)

Universal link between the boson peak and transverse phonons in glass journal October 2008
Membrane protein megahertz crystallography at the European XFEL journal November 2019
Ultracompact 3D microfluidics for time-resolved structural biology journal January 2020
Serial protein crystallography in an electron microscope journal February 2020
High-speed protein crystallography journal October 2018
Time-resolved serial femtosecond crystallography at the European XFEL journal November 2019
MHz data collection of a microcrystalline mixture of different jack bean proteins journal April 2019
Double-pulse speckle contrast correlations with near Fourier transform limited free-electron laser light using hard X-ray split-and-delay journal March 2020
Megahertz single-particle imaging at the European XFEL journal May 2020
3D printed nozzles on a silicon fluidic chip journal March 2019
Spatial and temporal pre-alignment of an X-ray split-and-delay unit by laser light interferometry journal April 2019
Advances and opportunities in ultrafast X-ray crystallography and ultrafast structural optical crystallography of nuclear and electronic protein dynamics journal September 2019
Evaluation of serial crystallographic structure determination within megahertz pulse trains journal November 2019
Budgeting the emittance of photoemitted electron beams in a space-charge affected emission regime for free-electron laser applications journal March 2020
Thermomechanical response of thickly tamped targets and diamond anvil cells under pulsed hard x-ray irradiation journal May 2020
Controlled beams of shock-frozen, isolated, biological and artificial nanoparticles journal March 2020
X-rays put molecules into a spin journal February 2019
An outlook on using serial femtosecond crystallography in drug discovery journal May 2019
Scaling Laws of an Exploding Liquid Column under an Intense Ultrashort X-Ray Pulse journal August 2019
3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating journal August 2019
DatView : a graphical user interface for visualizing and querying large data sets in serial femtosecond crystallography journal October 2019
The Adaptive Gain Integrating Pixel Detector at the European XFEL journal January 2019
A versatile liquid-jet setup for the European XFEL journal February 2019
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation journal April 2019
The Karabo distributed control system journal August 2019
XANES and EXAFS of dilute solutions of transition metals at XFELs journal August 2019
Linear array detector for online diagnostics of spectral distributions at MHz repetition rates journal September 2019
XGANDALF – extended gradient descent algorithm for lattice finding journal August 2019
Sample delivery for serial crystallography at free-electron lasers and synchrotrons journal January 2019
A guide to sample delivery systems for serial crystallography journal August 2019
Initial observations of the femtosecond timing jitter at the European XFEL journal January 2019
3D printed nozzles on a silicon fluidic chip text January 2019
Membrane protein megahertz crystallography at the European XFEL text January 2019
Serial protein crystallography in an electron microscope text January 2020
The Adaptive Gain Integrating Pixel Detector at the European XFEL text January 2019
Spatial and temporal pre-alignment of an X-ray split-and-delay unit by laser light interferometry text January 2019
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation text January 2019
Initial observations of the femtosecond timing jitter at the European XFEL text January 2019
XGANDALF – extended gradient descent algorithm for lattice finding text January 2019
Linear Array Detector for Online Diagnostics of Spectral Distributions at MHz Repetition Rates text January 2019
Membrane protein megahertz crystallography at the European XFEL text January 2019
Time-resolved serial femtosecond crystallography at the European XFEL text January 2019
Evaluation of serial crystallographic structure determination within megahertz pulse trains text January 2019
The Karabo distributed control system text January 2019
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020
Serial protein crystallography in an electron microscope text January 2020
MHz Data Collection of a Microcrystalline mMxture of Different Jack Bean Proteins text January 2019
Double-pulse speckle contrast correlations with near Fourier transform limited free-electron laser light using hard split-and-delay text January 2020
Budgeting the Emittance of Photoemitted Electron Beams in a Space-Charge affected Emission Regime for Free-Electron Laser Applications text January 2020
Megahertz single-particle imaging at the European XFEL text January 2020
New Trends in Protein Crystallization and Protein Crystallography journal January 2020
Thermomechanical response of thickly tamped targets and diamond anvil cells under pulsed hard x-ray irradiation text January 2018
Linear array detector for online diagnostics of spectral distributions at MHz repetition rates text January 2019

Figures / Tables (8)

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