Membrane protein megahertz crystallography at the European XFEL

Gisriel, Chris; Coe, Jesse; Letrun, Romain; Yefanov, Oleksandr M.; Luna-Chavez, Cesar; Stander, Natasha E.; Lisova, Stella; Mariani, Valerio; Kuhn, Manuela; Aplin, Steve; Grant, Thomas D.; Dörner, Katerina; Sato, Tokushi; Echelmeier, Austin; Cruz Villarreal, Jorvani; Hunter, Mark S.; Wiedorn, Max O.; Knoska, Juraj; Mazalova, Victoria; Roy-Chowdhury, Shatabdi; Yang, Jay-How; Jones, Alex; Bean, Richard; Bielecki, Johan; Kim, Yoonhee; Mills, Grant; Weinhausen, Britta; Meza, Jose D.; Al-Qudami, Nasser; Bajt, Saša; Brehm, Gerrit; Botha, Sabine; Boukhelef, Djelloul; Brockhauser, Sandor; Bruce, Barry D.; Coleman, Matthew A.; Danilevski, Cyril; Discianno, Erin; Dobson, Zachary; Fangohr, Hans; Martin-Garcia, Jose M.; Gevorkov, Yaroslav; Hauf, Steffen; Hosseinizadeh, Ahmad; Januschek, Friederike; Ketawala, Gihan K.; Kupitz, Christopher; Maia, Luis; Manetti, Maurizio; Messerschmidt, Marc; Michelat, Thomas; Mondal, Jyotirmoy; Ourmazd, Abbas; Previtali, Gianpietro; Sarrou, Iosifina; Schön, Silvan; Schwander, Peter; Shelby, Megan L.; Silenzi, Alessandro; Sztuk-Dambietz, Jolanta; Szuba, Janusz; Turcato, Monica; White, Thomas A.; Wrona, Krzysztof; Xu, Chen; Abdellatif, Mohamed H.; Zook, James D.; Spence, John C.  H.; Chapman, Henry N.; Barty, Anton; Kirian, Richard A.; Frank, Matthias; Ros, Alexandra; Schmidt, Marius; Fromme, Raimund; Mancuso, Adrian P.; Fromme, Petra; Zatsepin, Nadia A.

doi:10.1038/s41467-019-12955-3

Title: Membrane protein megahertz crystallography at the European XFEL

Journal Article · Mon Nov 04 00:00:00 EST 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-12955-3· OSTI ID:1596363

; Coe, Jesse;

; Yefanov, Oleksandr M.;

; Stander, Natasha E.; Lisova, Stella; Mariani, Valerio; Kuhn, Manuela; Aplin, Steve;

; Dörner, Katerina; Sato, Tokushi;

;

; Hunter, Mark S.; Wiedorn, Max O.; Knoska, Juraj;

; Roy-Chowdhury, Shatabdi more »

The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 Å-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); Univ. of Wisconsin, Milwaukee, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); European Research Council (ERC); German Research Foundation (DFG); German Federal Ministry of Education and Research (BMBF); Australian Research Council (ARC); USDOE National Nuclear Security Administration (NNSA)

Contributing Organization:: European XFEL Group

Grant/Contract Number:: AC02-76SF00515; SC0002164; SC0010575; R01GM095583; 609920; DFG-EXC1074; 05K18CHA; AC52-07NA27344; R01GM117342; CE140100011

OSTI ID:: 1596363

Alternate ID(s):: OSTI ID: 1598207; OSTI ID: 1959525

Report Number(s):: LLNL-JRNL-843428; TRN: US2102069

Journal Information:: Nature Communications, Vol. 10, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 34 works

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