Phyllosphere exudates select for distinct microbiome members in sorghum epicuticular wax and aerial root mucilage

Mechan, Marco; Mullet, John; Shade, Ashley

doi:10.1094/pbiomes-08-22-0046-fi

Phyllosphere exudates select for distinct microbiome members in sorghum epicuticular wax and aerial root mucilage

Journal Article · Sat Jan 07 04:00:00 EST 2023 · Phytobiomes Journal

DOI:https://doi.org/10.1094/pbiomes-08-22-0046-fi· OSTI ID:1922611

Mechan, Marco ^[1]; Mullet, John ^[2]; Shade, Ashley ^[3]

Michigan State Univ., East Lansing, MI (United States); Michigan State University Department of Plant, Soil and Microbial Sciences
Michigan State Univ., East Lansing, MI (United States); Texas A & M Univ., College Station, TX (United States)
Michigan State Univ., East Lansing, MI (United States); Univ. Lyon, Ecully cedex (France)

Phyllosphere exudates create specialized microhabitats that shape microbial community diversity. We explored the microbiome associated with two sorghum phyllosphere exudates, the epicuticular wax and aerial root mucilage. We assessed the microbiome associated with the wax from sorghum plants over two growth stages, and the root mucilage additionally from nitrogen-fertilized and non-fertilized plants. In parallel, we isolated and characterized hundreds of bacteria from wax and mucilage, and integrated data from cultivation-independent and cultivation-dependent approaches to gain insights into exudate diversity and bacterial phenotypes. We found that Sphingomonadaceae and Rhizobiaceae families were the major taxa in the wax regardless of water availability and plant developmental stage to plants. The cultivation-independent mucilage-associated bacterial microbiome contained Erwiniaceae, Flavobacteriaceae, Rhizobiaceae, Pseudomonadaceae, Sphingomonadaceae, and its structure was strongly influenced by sorghum development but only modestly influenced by fertilization. In contrast, the fungal community structure of mucilage was strongly affected by the year of sampling but not by fertilization or plant developmental stage, suggesting a decoupling of fungal-bacterial dynamics in the mucilage. Our bacterial isolate collection from wax and mucilage had several isolates that matched 100% to detected amplicon sequence variants, and were enriched on media that selected for phenotypes including phosphate solubilization, putative diazotrophy, resistance to desiccation, capability to grow on methanol as a carbon source, and ability to grow in the presence of linalool and β-caryophyllene (terpenes in sorghum wax). This work expands our understanding of the microbiome of phyllosphere exudates and supports our long-term goal to translate microbiome research to support sorghum cultivation.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409; FC02-07ER64494

OSTI ID:: 1922611

Journal Information:: Phytobiomes Journal, Journal Name: Phytobiomes Journal Journal Issue: 2 Vol. 7; ISSN 2471-2906

Publisher:: American Phytopathological Society (APS)Copyright Statement

Country of Publication:: United States

Language:: English

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