Galactomannan utilization by Cellvibrio japonicus relies on a single essential α‐galactosidase encoded by the aga27A gene

Novak, Jessica K.; Gardner, Jeffrey G.

doi:10.1111/mmi.15024

Title: Galactomannan utilization by Cellvibrio japonicus relies on a single essential α‐galactosidase encoded by the aga27A gene

Journal Article · Fri Jan 20 00:00:00 EST 2023 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.15024· OSTI ID:1914357

Novak, Jessica K. ^[1];

^[1]

Department of Biological Sciences University of Maryland – Baltimore County Baltimore Maryland USA

Abstract Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side‐chain substitutions. Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose. One bacterium that possesses this suite of enzymes is the Gram‐negative saprophyte Cellvibrio japonicus , which has 10 predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27. Here we describe a systems biology approach to identify and characterize the essential mannan‐degrading components in this bacterium. The transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode carbohydrate active enzymes (CAZymes) when mannan was actively being degraded. A comprehensive mutational analysis characterized 54 CAZyme‐encoding genes in the context of mannan utilization. Growth analysis of the mutant strains found that the man26C , aga27A , and man5D genes, which encode a mannobiohydrolase, α‐galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential. Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.

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Sponsoring Organization:: USDOE

OSTI ID:: 1914357

Journal Information:: Molecular Microbiology, Journal Name: Molecular Microbiology Vol. 119 Journal Issue: 3; ISSN 0950-382X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: FAO

Language:: English

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