Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification

Nelson, Cassandra E.; Attia, Mohamed A.; Rogowski, Artur; Morland, Carl; Brumer, Harry; Gardner, Jeffrey G.

doi:10.1111/1462-2920.13959

Title: Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification

Journal Article · Fri Oct 20 00:00:00 EDT 2017 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.13959· OSTI ID:1406244

Nelson, Cassandra E. ^[1]; Attia, Mohamed A. ^[2]; Rogowski, Artur ^[3]; Morland, Carl ^[3]; Brumer, Harry ^[2];

^[1]

Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
Univ. of British Columbia, Vancouver (Canada)
Newcastle Univ., Newcastle Upon Tyne (United Kingdom)

Here, lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1!3)/β(1!4) mixed-linkage glucan (MLG), and β(1!3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has a robust capacity for plant polysaccharide degradation, due to a genome encoding a large contingent of Carbohydrate-Active Enzymes (CAZymes), many of whose specific functions remain unknown. Using a comprehensive genetic and biochemical approach we have delineated the physiological roles of the four C. japonicus Glycoside Hydrolase Family 3 (GH3) members on diverse β-glucans. Despite high protein sequence similarity and partially overlapping activity profiles on disaccharides, these β-glucosidases are not functionally equivalent. Bgl3A has a major role in MLG and sophorose utilization, and supports β(1!3) glucan utilization, while Bgl3B underpins cellulose utilization and supports MLG utilization. Bgl3C drives β(1!3) glucan utilization. Finally, Bgl3D is the crucial β-glucosidase for XyG utilization. This study not only sheds the light on the metabolic machinery of C. japonicus, but also expands the repertoire of characterized CAZymes for future deployment in biotechnological applications. In particular, the precise functional analysis provided here serves as a reference for informed bioinformatics on the genomes of other Cellvibrio and related species.

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Research Organization:: Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0014183

OSTI ID:: 1406244

Journal Information:: Environmental Microbiology, Vol. 19; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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09 BIOMASS FUELS
β-glucosidase
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lignocellulose
mixed-linkage glucan
xyloglucan