Biochemical and spectroscopic characterizations of the oligomeric antenna of the coral symbiotic&nbsp;Symbiodiniaceae Fugacium kawagutii

Niedzwiedzki, Dariusz M.; Magdaong, Nikki Cecil M.; Su, Xinyang; Liu, Haijun

doi:10.1007/s11120-022-00951-6

Biochemical and spectroscopic characterizations of the oligomeric antenna of the coral symbiotic Symbiodiniaceae Fugacium kawagutii

Journal Article · Wed Sep 07 00:00:00 EDT 2022 · Photosynthesis Research

DOI:https://doi.org/10.1007/s11120-022-00951-6· OSTI ID:1908353

Niedzwiedzki, Dariusz M. ^[1]; Magdaong, Nikki Cecil M. ^[2]; Su, Xinyang ^[2]; ^[2]

Washington Univ., St. Louis, MO (United States); Washington University in St. Louis
Washington Univ., St. Louis, MO (United States)

Light-harvesting antennas in photosynthesis capture light energy and transfer it to the reaction centers (RCs) where photochemistry takes place. The sustainable growth of the reef-building corals relies on a constant supply of the photosynthates produced by the endosymbiotic dinoflagellate, belonging to the family of Symbiodiniaceae. The antenna system in this group consists of the water-soluble peridinin-chlorophyll $$\textit{a}$$-protein (PCP) and the intrinsic membrane chlorophyll $$\textit{a}$$-chlorophyll $$c_2$$-peridinin protein complex (acpPC). In this report, a nonameric acpPC is reported in a dinoflagellate, Fugasium kawagutii (formerly Symbiodinium kawagutii sp. CS-156). We found that extensive biochemical purification altered the oligomerization states of the initially isolated nonameric acpPC. The excitation energy transfer pathways in the acpPC nonamer and its variants were studied using time-resolved fluorescence and time-resolved absorption spectroscopic techniques at 77 K. Compared to the well-characterized trimeric acpPC, the nonameric acpPC contains an 11 nm red-shifted terminal energy emitter and substantially altered excited state lifetimes of Chl $$\textit{a}$$. The observed energetic overlap of the fluorescence terminal energy emitters with the absorption of RCs is hypothesized to enable efficient downhill excitation energy transfer. Additionally, the shortened Chl $$\textit{a}$$ fluorescence decay lifetime in the oligomeric acpPC indicate a protective self-relaxation strategy. We propose that the highly-oligomerized acpPC nonamer represents an intact functional unit in the Symbiodiniaceae thylakoid membrane. They perform efficient excitation energy transfer (to RCs), and are under manageable regulations in favor of photoprotection.

View Accepted Manuscript (DOE)

Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-07ER15902

OSTI ID:: 1908353

Journal Information:: Photosynthesis Research, Journal Name: Photosynthesis Research Journal Issue: 2 Vol. 154; ISSN 0166-8595

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Biochemical and spectroscopic characterizations of the oligomeric antenna of the coral symbiotic Symbiodiniaceae Fugacium kawagutii

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