Evidence of functional trimeric chlorophyll a/c-peridinin proteins in the dinoflagellate Symbiodinium

Jiang, Jing; Zhang, Hao; Orf, Gregory S.; Lu, Yue; Xu, Wenxin; Harrington, Lucas B.; Liu, Haijun; Lo, Cynthia S.; Blankenship, Robert E.

doi:10.1016/j.bbabio.2014.07.023

Evidence of functional trimeric chlorophyll a/c-peridinin proteins in the dinoflagellate Symbiodinium

Journal Article · Sat Nov 01 04:00:00 EDT 2014 · Biochimica et Biophysica Acta - Bioenergetics

DOI:https://doi.org/10.1016/j.bbabio.2014.07.023· OSTI ID:1150716

Jiang, Jing ^[1]; Zhang, Hao; ; Lu, Yue; Xu, Wenxin; Harrington, Lucas B.; ; Lo, Cynthia S.; Blankenship, Robert E.

Washington University in St. Louis

The chlorophyll a-chlorophyll c2-peridinin-protein (apcPC), a major light harvesting component in peridinin-containing dinoflagellates, is an integral membrane protein complex. We isolated functional acpPC from the dinoflagellate Symbiodinium. Both SDS-PAGE and electrospray ionization mass spectrometry (ESI-MS) analysis quantified the denatured subunit polypeptide molecular weight (MW) as 18kDa. Size-exclusion chromatography (SEC) and blue native gel electrophoresis (BN-PAGE) were employed to estimate the size of native acpPC complex to be 64-66kDa. We also performed native ESI-MS, which can volatilize and ionize active biological samples in their native states. Our result demonstrated that the native acpPC complex carried 14 to 16 positive charges, and the MW of acpPC with all the associated pigments was found to be 66.5kDa. Based on these data and the pigment stoichiometry, we propose that the functional light harvesting state of acpPC is a trimer. Our bioinformatic analysis indicated that Symbiodinium acpPC shares high similarity to diatom fucoxanthin Chl a/c binding protein (FCP), which tends to form a trimer. Additionally, acpPC protein sequence variation was confirmed by de novo protein sequencing. Its sequence heterogeneity is also discussed in the context of Symbiodinium eco-physiological adaptations.

Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

DOE Contract Number:: SC0001035

OSTI ID:: 1150716

Report Number(s):: DE-SC0001035-122

Journal Information:: Biochimica et Biophysica Acta - Bioenergetics, Journal Name: Biochimica et Biophysica Acta - Bioenergetics Journal Issue: 11 Vol. 1837; ISSN 0005-2728

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

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