Differences in the protein profiles of cultured and endosymbiotic symbiodinium sp. (pyrrophyta) from the anemone aiptasia pallida (anthozoa)

Journal Article · Sat Feb 01 04:00:00 EST 1997 · Journal of Phycology

DOI:https://doi.org/10.1111/j.0022-3646.1997.00044.x· OSTI ID:478379

Stochaj, W R; Grossman, A R ^[1]

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1. Carnegie Institution of Washington, Stanford, CA (United States)

One- and two-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunological analyses were used to visualize differences in polypeptides synthesized by Symbiodinium sp. from the anemone Aiptasia pallida when grown in the cultured and endosymbiotic states (freshly isolated zooxanthellae). Surprisingly, a comparison of proteins in cultured and endosymbiotic Symbiodinium sp. revealed only four major polypeptides with similar isoelectric and molecular mass characteristics. Using monospecific antibodies, we demonstrated differences in specific proteins synthesized by the dinoflagellate in the two different growth states. The dimeric, 14 kDa form of the peripheral membrane peridinin-chlorophyll a binding protein predominates under endosymbiotic conditions, whereas the monomeric, 35 kDa form predominates under the culture conditions used in this study. Antibodies to form II ribulose-1,5-bisphosphate carboxylase revealed 62 and 60 kDa forms of this protein in the alga grown as an endosymbiont and in culture, respectively. Differences in the integral membrane peridinin-chlorophyll a-c-binding proteins were also observed. These results demonstrate that there are major changes in the populations of proteins synthesized by Symbiodinium sp. in response to the conditions in hospite. Such changes may reflect a developmental switch that tailors the physiology of the alga to the conditions encountered in the endosymbiotic state. 77 refs., 6 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 478379
• Journal Information: Journal of Phycology, Journal Name: Journal of Phycology Journal Issue: 1 Vol. 33; ISSN 0022-3646; ISSN JPYLAJ
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOCHEMISTRY
CNIDARIA
DINOFLAGELLATE
SYMBIOSIS